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OP14L Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6)

Anti-c-ErbB2/c-Neu (Ab-2), mouse monoclonal, clone 9G6, recognizes the ~185 kDa c-ErbB2/c-Neu SK-BR-3 cells and breast carcinoma. It is validated for IP and IHC on frozen and paraffin sections.

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus

OP14L
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP14L-100UGCN Glass bottle 100 μg
Description
OverviewRecognizes the ~185 kDa c-ErbB2/c-Neu in SK-BR-3 cells and breast carcinoma tissue. Does not cross-react with v-ErbB2 or the EGF receptor.
Catalogue NumberOP14L
Brand Family Calbiochem®
SynonymsAnti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
References
ReferencesVan De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239.
DiFiore, P.P., et al.1987 Science 237, 178.
Slamon, D.J., et al. 1987 Science 235, 177.
Varley, J.M., et al.1987 Oncogene 1, 423.
Bargmann, C.I., et al. 1986 Nature 319, 226.
Yamamoto, T., et al.1986 Nature 319, 230.
Blick, M., et al. 1984 Blood 64, 1234.
Product Information
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
Negative controlHepG2 cells or normal skin
Positive controlSK-BR-3 cells or breast carcinoma tissue
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesParaffin Sections Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
Key Applications Frozen Sections
Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (5 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (see application references)
Application CommentsImmunoprecipitates the ~185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein. This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogencells overexpressing human c-ErbB2/c-Neu
ImmunogenHuman
Clone9G6
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP14L-100UGCN 04055977224825

Documentation

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6) SDS

Title

Safety Data Sheet (SDS) 

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6) Certificates of Analysis

TitleLot Number
OP14L

References

Reference overview
Van De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239.
DiFiore, P.P., et al.1987 Science 237, 178.
Slamon, D.J., et al. 1987 Science 235, 177.
Varley, J.M., et al.1987 Oncogene 1, 423.
Bargmann, C.I., et al. 1986 Nature 319, 226.
Yamamoto, T., et al.1986 Nature 319, 230.
Blick, M., et al. 1984 Blood 64, 1234.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2007 RFH
SynonymsAnti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
ApplicationFrozen Sections (5 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (see application references)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 mouse myeloma cells. Recognizes the ~185 kDa c-ErbB2/c-neu protein.
BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways, and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal growth factor Receptor) and is the second member of the EGF receptor family to be described.
HostMouse
Immunogen speciesHuman
Immunogencells overexpressing human c-ErbB2/c-Neu
Clone9G6
IsotypeIgG₁
Specieshuman
Positive controlSK-BR-3 cells or breast carcinoma tissue
Negative controlHepG2 cells or normal skin
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
PreservativeNone
CommentsImmunoprecipitates the ~185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein. This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesVan De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239.
DiFiore, P.P., et al.1987 Science 237, 178.
Slamon, D.J., et al. 1987 Science 235, 177.
Varley, J.M., et al.1987 Oncogene 1, 423.
Bargmann, C.I., et al. 1986 Nature 319, 226.
Yamamoto, T., et al.1986 Nature 319, 230.
Blick, M., et al. 1984 Blood 64, 1234.
Application referencesParaffin Sections Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.