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ABN1647 Anti-VGluT1

Anti-VGLUT1, Cat. No. ABN1647, is a highly specific rabbit polyclonal antibody that targets Vesicular glutamate transporter 1 and has been tested in Immunoautoradiography, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Anti-VGluT1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Slc17a7, Bnpi, Vglut1 UniProt Number: Q62634
ABN1647
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, R, HIF, IHC, Immunoautoradiography, WBRbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABN1647
DescriptionAnti-VGluT1
Alternate Names
  • Vesicular glutamate transporter 1
  • Brain-specific Na(+)-dependent inorganic phosphate cotransporter
  • Solute carrier family 17 member 7
Background InformationVesicular glutamate transporter 1 (UniProt: also known as VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7) is encoded by the Slc17a7 (also known as Bnpi, Vglut1) gene (Gene ID: 116638) in rat. VGLUT1 is a multi-pass synaptic vesicle membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neuronal cells. Glutamate transporters mediate glutamate uptake into synaptic vesicles and are driven by a proton electrochemical gradient generated by the vacuolar H+-ATPase. Two isoforms of VGLUT1 have been reported that are produced by alternative splicing. VGLUT1 is expressed in the cerebellum, cerebral cortex and hippocampus. Expression of isoform VGLUT2 increases sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. It has been shown that both VGLUT1 and VGLUT2 are expressed prominently in the adult brain, however, during the first two weeks of postnatal development, VGLUT2 expression predominates. VGLUT1 expression becomes essential for survival at the time of the developmental switch from VGLUT2 to VGLUT1. The VGLUT1 knockout mice die at the age when a strong upregulation of VGLUT1 through newly emerging VGLUT1 synapses as well as a replacement of the previously predominant VGLUT2 isoform in cerebellar, hippocampal, and cortical neurons occurs. (Ref.: Wojcik, SM et al. (2004). Proc. Natl. Acad. Sci. USA 101(18): 7158-7163).
References
Product Information
FormatUnpurified
HS Code3002 15 90
PresentationRabbit polyclonal antiserum with 0.05% sodium azide and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-VGLUT1, Cat. No. ABN1647, is a highly specific rabbit polyclonal antibody that targets Vesicular glutamate transporter 1 and has been tested in Immunoautoradiography, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunohistochemistry
  • Immunoautoradiography
  • Western Blotting
Application NotesImmunoautoradiography Analysis: A representative lot detected VGLUT1 in rat and mouse striatum and hippocampus tissue (Courtesy of Dr.Salah el mestikawy PhD, at DR1 CNRS (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQU - SATT LUTECH).

Immunohistochemistry Analysis: A representative lot detected VGLUT1 in prefrontal cortex of a representative control subject and AD patient (Kashani, A., et. al. (2008). Neurobiol Aging. 29(11):1619-30).

Western Blotting Analysis: A representative lot detected VGLUT1 in glutamate transporter 1 in rat and human putamen extracts (Kashani, A., et. al. (2007). Neurobiol Agining 28(4):568-78) and prefrontal cortex of controls, MCI, early stage Alzheimer (Kashani, A., et. al. (2008). Neurobiol Aging. 29(11):1619-30).

Immunofluorescence Analysis: A representative lot detected VGLUT1 in mouse striatum and hippocampus tissue (Courtesy of Dr.Salah el mestikawy PhD, at DR1 CNRS (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQU - SATT LUTECH).

Immunofluorescence Analysis: A representative lot detected VGLUT1 in rat striatum (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).

Immunoautoradiography Analysis: A representative lot detected VGLUT1 in coronal sections of frontal rat brain (Herzog, E., et. al. (2004). Neuroscience. 123(4):983-1002) and rat brain sagittal sections (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).

Western Blotting Analysis: A representative lot detected VGLUT1 in BON cells stably expressing VGLUT1 (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of rat Vesicular glutamate transporter 1 (VGLUT1).
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Vesicular glutamate transporter 1 in Human, Mouse, and Rat brain. It targets an epitope with in 14 amino acids from the C-terminal region.
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
  • Human
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Bovine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Slc17a7
  • Bnpi
  • Vglut1
Purification MethodUnpurified
UniProt Number
Molecular Weight~55 kDa observed; 61.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected VGLUT1 in 20 µg of mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN1647 04054839315961