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DR1047 Anti-UHRF1 Mouse mAb (3A11)

DR1047
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
DR1047-100UGCN 100 μg
Description
OverviewRecognizes the ~90 kDa UHRF1 protein in HeLa cells and human endometrium tissue.
Catalogue NumberDR1047
Brand Family Calbiochem®
SynonymsAnti-Ubiquitin-Like PHD/RING Finger Domain 1
Application Data
Detection of human UHRF1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human UHRF1 by staining paraffin sections. Sample: Formalin-fixed, paraffin embedded human endometrium tissue. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (3 µg/ml). Detection: colorimetric (DAB).

Detection of human UHRF1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (10 µg/ml). Detection: fluorescence.
References
ReferencesMistry H, et al. 2008. Biochem. Biophys. Res. Commun. 375 321.
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells, Human endometrium tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acides 694-794 of human rUHRF1, expressed as a GST fusion protein
ImmunogenNA
Clone3A11
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
DR1047-100UGCN 04055977225969