Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABD119 Anti-TLE1 Antibody

This Anti-TLE1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of TLE1.

Anti-TLE1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Histone Modifying Proteins Gene Symbol: TLE1 UniProt Number: Q04724
ABD119
100 μg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABD119
DescriptionAnti-TLE1 Antibody
Alternate Names
  • Transducin-like enhancer protein 1
  • E(Sp1) homolog
  • Enhancer of split groucho-like protein 1
  • ESG1
Background InformationTransducin-like enhancer protein 1 (UniProt Q04724; also known as E(Sp1) homolog, Enhancer of split groucho-like protein 1, ESG1) is encoded by the TLE1 gene (Gene ID 7088) in human. TLE proteins interact with HDACs as well as deacetylated histone tails and play an important role in transcription regulation in several signaling pathways, including Ras, Notch, and Wnt. TLE proteins contain a conserved N-terminal glutamine-rich (Q) domain (a.a. 1-131 of TLE1) that mediates TLEs tetramerization as well as TCF/LEF proteins binding, and is essential for chromatin oligomerization. This is followed by a variable central domain sub-divided into a glycine-prolin-rich (GP) region (a.a. 132-199 of TLE1), the CcN (protein kinase CK2/cell cycle-dependent kinase 2/nuclear localization sequence) domain (a.a. 200-268), a serine-proline-rich (SP) region (a.a. 200-268 and 269-450 of TLE1), and a C-terminal WD40 domain with 6 WD repeats (a.a. 470-767 of TLE1) that interact with multiple transcription factors. The GP region binds directly to HDAC1, the CcN region contains consensus CK2 and Cdc2 phosphorylation sites that are modified in a cell cycle-dependent manner, the SP region is phosphorylated by the Ser/Thr kinases HIPK2 and MAPK, which appear to negatively regulate transcription repression activity, and may also interact with basic helix-loop-helix transcription factors. The C-terminal WD40 domain is essential for chromatin condensation and, together with SP, interacts with bHLH proteins.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-TLE1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of TLE1.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 1 µg/mL from a representative lot detected TLE1 in HeLa cells and H9 human stem cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a sequence within the CcN domain of human TLE1.
EpitopeCcN domain.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody targets the CcN (protein kinase CK2/cell cycle-dependent kinase 2/nuclear localization sequence) domain of TLE1 and stained cell nuclei by fluorescent immunocytochemistry.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TLE1
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~90 kDa observed. 83.20 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in H9 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antiobdy detected TLE1 in 10 µg of H9 human stem cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABD119 04055977352832