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573822 Anti-Synaptophysin Mouse mAb (SP15)

This Anti-Synaptophysin Mouse mAb (SP15) is validated for use in Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of Synaptophysin.

Anti-Synaptophysin Mouse mAb (SP15) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
573822
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Fe, Ht, H, M, Porcine, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
573822-50ULCN Glass bottle 50 ul
Description
OverviewRecognizes the ~38 kDa synaptophysin protein in brain tissue. Does not cross-react with the ~65 kDa form in mouse liver extracts.
Catalogue Number573822
Brand Family Calbiochem®
Application Data
Detection of rat synaptophysin by immunoblotting. Sample: Whole tissue extract from rat liver. Primary antibody: Anti-Synaptophysin Mouse mAb (SP15) (Cat. No. 573822) (1:10,000). Detection: chemiluminescence.
References
ReferencesAlder, J., et al. 1995. J. Neurosci. 15, 511.
Thomas, L., et al. 1988. Science 242, 1050.
Rehmm, H., et al. 1986. EMBO J. 5, 5353.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Negative controlMouse liver extract
Positive controlRat liver tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesFrozen Sections Honer, W.G., et al. 1993. Brain Res. 609, 9.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesFrozen Sections (1:10; see application references)
Immunoblotting (1:2000, colorimetric)
Immunocytochemistry (1:250)
Immunoprecipitation (1:100)
Application CommentsVariables associated with assay conditions will dictate the proper working dilutions.
Biological Information
Immunogensynaptic vesicle-containing fractions from human brain homogenates
ImmunogenHuman
CloneSP15
HostMouse
IsotypeIgM
Species Reactivity
  • Bovine
  • Feline
  • Hamster
  • Human
  • Mouse
  • Porcine
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
573822-50ULCN 04055977189544

Documentation

Anti-Synaptophysin Mouse mAb (SP15) Certificates of Analysis

TitleLot Number
573822

References

Reference overview
Alder, J., et al. 1995. J. Neurosci. 15, 511.
Thomas, L., et al. 1988. Science 242, 1050.
Rehmm, H., et al. 1986. EMBO J. 5, 5353.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-February-2008 JSW
ApplicationFrozen Sections (1:10; see application references)
Immunoblotting (1:2000, colorimetric)
Immunocytochemistry (1:250)
Immunoprecipitation (1:100)
Application Data
Detection of rat synaptophysin by immunoblotting. Sample: Whole tissue extract from rat liver. Primary antibody: Anti-Synaptophysin Mouse mAb (SP15) (Cat. No. 573822) (1:10,000). Detection: chemiluminescence.
DescriptionAmmonium sulfate purified (50%) mouse monoclonal antibody. Recognizes the ~38 kDa syaptophysin protein.
BackgroundSynaptophysin is a ~38 kDa integral membrane protein on synaptic vesicles. This protein consists of four transmembrane domains, with its amino- and carboxyl-terminus facing the cytoplasm. Due to its transmembrane domain structure, synaptophysin has been postulated to be a gap junction-like protein as voltage-dependent channel activity is seen when synaptophysin hexamers are reconstituted into lipid membranes. Physiological evidence for synaptophysin’s role in secretion includes its involvement in calcium-dependent glutamate secretion in Xenopus oocytes injected with rat cerebellum mRNA.
HostMouse
Immunogen speciesHuman
Immunogensynaptic vesicle-containing fractions from human brain homogenates
CloneSP15
IsotypeIgM
Speciesbovine, feline, hamster, human, mouse, porcine, rat
Positive controlRat liver tissue
Negative controlMouse liver extract
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Preservative≤0.1% sodium azide
CommentsVariables associated with assay conditions will dictate the proper working dilutions.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesAlder, J., et al. 1995. J. Neurosci. 15, 511.
Thomas, L., et al. 1988. Science 242, 1050.
Rehmm, H., et al. 1986. EMBO J. 5, 5353.
Application referencesFrozen Sections Honer, W.G., et al. 1993. Brain Res. 609, 9.