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MABS1320-25UL Anti-Serpin B13 Antibody, clone B29

Anti-Serpin B13, clone B29, Cat. No. MABS1320, is a mouse monoclonal antibody that detects Serpin B13 and has been tested for use in Function Studies, Immunofluorescence, and Western Blotting.

Anti-Serpin B13 Antibody, clone B29 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Serpinb13 UniProt Number: Q8CDC0
MABS1320-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MFunction Assay, IF, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1320-25UL
DescriptionAnti-Serpin B13 Antibody, clone B29
Background InformationSerpin B13 (UniProt: Q8CDC0) is encoded by the Serpinb13 gene (Gene ID: 241196) in murine species. Serpin B13 is a cytoplasmic protein that plays a role in the proliferation or differentiation of keratinocytes. Two isoforms of Serpin B13 have been described that are generated by alternative splicing. Serpin B13 acts as a serine proteinase inhibitor and studies have shown a strong induction of serpin B13 in the pancreas of mice during the first few weeks of life. Cathepsin L and K appear to be the targets of serpin B13 action. Serpin B13 is expressed in pancreas with low levels of expression in pancreatic islets and larger expression in the exocrine pancreas. Secretion of anti-serpin B13 autoantibodies in young diabetes-prone non-obese diabetic (NOD) mice is shown to be associated with reduced inflammation in pancreatic islets and a slower progression to autoimmune diabetes. Anti-serpin B13 autoantibodies are shown to protect NOD mice against diabetes before 16 weeks of age. Reduced expression of serpin B13 autoantibodies is associated with development of type I diabetes in human before 5 years of age. Mice injected with monoclonal anti-serpin B13 display few inflammatory cells in the islets and rapid recovery from recent onset diabetes.
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Serpin B13, clone B29, Cat. No. MABS1320, is a mouse monoclonal antibody that detects Serpin B13 and has been tested for use in Function Studies, Immunofluorescence, and Western Blotting.
Key Applications
  • Function Assay
  • Immunofluorescence
  • Western Blotting
Application NotesImmunofluorescence Analysis: A representative lot detected Serpin B13 in Immunofluorescence applications (Baldzizhar, R., et. al. (2012). J Biol Chem. 288(3):1612-9).

Western Blotting Analysis: 3 ug/mL from a representative lot detected Serpin B13 in mouse serpin/HEK293 cell lysates (Courtesy of Dr. Jan Czyzyk, M.D., University of Rochester Medical Center, NY).

Western Blotting Analysis: A representative lot detected Serpin B13 in Western Blotting applications (Baldzizhar, R., et. al. (2012). J Biol Chem. 288(3):1612-9; Czyzyk, J., et. al. (2012). J Immunol. 188(12):6319-27).

Affects Function Analysis: A representative lot detected Serpin B13 in Affects Function applications (Baldzizhar, R., et. al. (2012). J Biol Chem. 288(3):1612-9).
Biological Information
ImmunogenPurified full length mouse Serpin B13 resuspended in Complete Freund's Adjuvant.
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone B29 detects Serpin B13 in human and murine species.
IsotypeIgG2b
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Serpinb13
Purification MethodProtein G purified
UniProt Number
Molecular Weight44.32 kda calculated.
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1κ. 
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Global Trade Item Number
Catalogue Number GTIN
MABS1320-25UL 04054839497100