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ABD38 Anti-SNAI1 (Snail homolog 1) Antibody

Anti-SNAI1 (Snail homolog 1) Antibody is a Rabbit Polyclonal Antibody for detection of SNAI1 also known as Zinc finger protein SNAI1, Snail homolog 1 & has been validated in WB.

Anti-SNAI1 (Snail homolog 1) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Transcription Factors Gene Symbol: SNAI1, SNAH UniProt Number: O95863
ABD38
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, R, HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABD38
DescriptionAnti-SNAI1 (Snail homolog 1) Antibody
Alternate Names
  • Zinc finger protein SNAI1
  • Protein snail homolog 1
  • Protein sna
Background InformationSNAI1, also known as Snail homolog 1, is a transcription factor that down regulates the expression of ectodermal genes within the mesoderm and is thought to be essential for mesoderm formation in the developing embryo. SNAI1 is expressed in the kidney and in mesenchymal and epithelial cell lines. Mutations in SNAI1 have been associated with tumors.
References
Product Information
FormatAffinity Purified
Control
  • Rat brain tissue lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SNAI1 (Snail homolog 1) Antibody is a Rabbit Polyclonal Antibody for detection of SNAI1 also known as Zinc finger protein SNAI1, Snail homolog 1 & has been validated in WB.
Key Applications
  • Western Blotting
Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected SNAI1 on 10 µg of mouse brain tissue lysate.
Biological Information
ImmunogenLinear peptide corresponding to human SNAI1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Mouse
  • Rat
  • Human
Species Reactivity NoteDemonstrated to react with Mouse and Rat. Predicted to react with Human based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008].
Gene Symbol
  • SNAI1
  • SNAH
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription.

SUBUNIT STRUCTURE: Interacts with FBXL14 and GSK3B. Interacts with BTRC; interaction occurs when it is phosphorylated on the destruction motif.

SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

TISSUE SPECIFICITY: Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.

PTM: Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination.

Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation.

SEQUENCE SIMILARITIES: Belongs to the snail C2H2-type zinc-finger protein family.

Contains 4 C2H2-type zinc fingers.
Molecular Weight~29 kDa observed. An uncharacterized band appears at ~40 kDa in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected SNAI1 on 10 µg of rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABD38 04053252437304

Documentation

Anti-SNAI1 (Snail homolog 1) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-SNAI1 (Snail homolog 1) Antibody Certificates of Analysis

TitleLot Number
Anti-SNAI1 (Snail homolog 1) - 2383574 2383574
Anti-SNAI1 (Snail homolog 1) - 2424800 2424800
Anti-SNAI1 (Snail homolog 1) - 2301275 2301275
Anti-SNAI1 (Snail homolog 1) - 2514269 2514269
Anti-SNAI1 (Snail homolog 1) - 3499834 3499834
Anti-SNAI1 (Snail homolog 1) - Q1970941 Q1970941
Anti-SNAI1 (Snail homolog 1) -2611667 2611667
Anti-SNAI1 (Snail homolog 1) -2783080 2783080