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MABF2804-25UG Anti-Reticular fibroblasts Antibody, clone ER-TR7

Anti-Reticular fibroblasts, clone ER-TR7, Cat. No. MABF2804, is a rat monoclonal antibody that detects Reticular fibroblasts and is tested for use in Immunofluorescence and Immunohistochemistry.

MABF2804-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberMABF2804-25UG
DescriptionAnti-Reticular fibroblasts Antibody, clone ER-TR7
Alternate Names
  • Fibroblast marker
Background InformationConnective tissue is responsible for maintaining the form of the body and its organs and provides cohesion and internal support. It includes several types of fibrous tissue that vary only in their density and cellularity. The cells of connective tissue include fibroblasts and adipose cells that are relatively stationary and several types of motile migrating cells, such as mast cells, macrophages, monocytes, lymphocytes, plasma cells, and eosinophils. These cells of connective tissue develop during embryonic life from the mesenchyme. Fibroblasts are the least specialized cells in the connective-tissue family. They are dispersed in connective tissue throughout the body, where they secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. In skin fibroblasts originate from two distinct lineage of cells: one lineage populates the papillary dermis, and the other lineage populates the reticular dermis, that have distinct cell markers and distinct functional abilities. Clone ER-TR specifically detects an antigen present in, and produced by, reticular fibroblasts. This clone is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. It is also shown to detect reticular fibroblasts in thymus. (Ref.: Van Vliet, E., et al. (1984). Eur. J. Immunol. 14(6); 524-529).
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Reticular fibroblasts, clone ER-TR7, Cat. No. MABF2804, is a rat monoclonal antibody that detects Reticular fibroblasts and is tested for use in Immunofluorescence and Immunohistochemistry.
Key Applications
  • Immunohistochemistry
  • Immunofluorescence
Application NotesTested applications

Immunofluorescence Analysis: A representative lot detected Reticular fibroblasts clone ER-TR7 in Immunofluorescence applications (Van Vliet, E., et al. (1984). Eur J Immunol. 14(6):524-9; Nolte, M.A., et al. (2003). J Exp Med. 198(3):505-12; Sixt, M., et al. (2005). Immunity. 22(1):19-29; Link, A., et al. (2007). Nat Immunol. 8(11):1255-65; Odaka, C., et al. (2009). J Histochem Cytochem. 57(4):373-82; Schiavinato, A., et al. (2021). Eur J Immunol. 51(9):2345-2347).

Immunohistochemistry Applications: A representative lot detected Reticular fibroblasts clone ER-TR7 in Immunohistochemistry applications (Van Vliet, E., et al. (1984). Eur J Immunol. 14(6):524-9; Nolte, M.A., et al. (2003). J Exp Med. 198(3):505-12; Sixt, M., et al. (2005). Immunity. 22(1):19-29; Link, A., et al. (2007). Nat Immunol.;8(11):1255-65; Odaka, C., et al. (2009). J Histochem Cytochem. 57(4):373-82; Schiavinato, A., et al. (2021). Eur J Immunol. 51(9):2345-2347).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenThymic stromal cells isolated from C3H mice.
EpitopeUnknown
CloneER-TR7
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone ER-TR7 is a rat monoclonal antibody that detects Reticular fibroblasts.
IsotypeIgG2a
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteMouse, Human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein G purified
UniProt Number
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in Mouse thymus tissue sections.

Immunohistochemistry Applications: A 1:50 dilution of this antibody detected reticular fibroblasts in frozen section of Mouse thymus tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Global Trade Item Number
Catalogue Number GTIN
MABF2804-25UG 04065268900901