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MABN1598 Anti-RetGC-1/GUCY2D Antibody, clone 6D8.2

This Anti-RetGC-1 Antibody, clone 6D8.2 is validated for use in Western Blotting for the detection of GUCY2D.

Anti-RetGC-1/GUCY2D Antibody, clone 6D8.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Sensory & PNS Gene Symbol: GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1 UniProt Number: Q02846
MABN1598
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1598
DescriptionAnti-RetGC-1/GUCY2D Antibody, clone 6D8.2
Alternate Names
  • Retinal guanylyl cyclase 1
  • Guanylate cyclase 2D, retinal
  • RETGC-1
  • Rod outer segment membrane guanylate cyclase
  • ROS-GC
Background InformationRetinal guanylyl cyclase 1 (EC 4.6.1.2; UniProt Q02846; also known as Guanylate cyclase 2D, retinal, RETGC-1, Rod outer segment membrane guanylate cyclase, ROS-GC) is encoded by the GUCY2D (also known as CORD6, GUC1A4, GUC2D, RETGC, RETGC1) gene (Gene ID 3000) in human. RetGC-1 is a membrane-bound retinal guanylyl cyclase expressed predominantly in the cone and rod photoreceptors in the cone outer segment. RetGC-1 and the related RetGC-2 mediate the synthesis of cyclic 3′, 5′-guanosine monophosphate (cGMP) from guanosine triphosphate in mammalian photoreceptor cells, where RetGC-1 and its associated activator proteins are responsible for the Ca2+-sensitive restoration of cGMP levels after light activation of the phototransduction cascade. RetGC-1 is produced with a signal peptide (a.a. 1-51) sequence, the removal of which yields the mature enzyme with an exracellular domain (a.a. 52-462), followed by a transmembrane segment (a.a. 463-487) and a cytoplasmic tail (a.a. 488-1103) that contains a protein kinase homology domain (a.a. 525-808), a dimerization domain, and the guanylate cyclase catalytic domain (a.a. 880-1010). Heterozygous mutations in the GUCY2D gene have been shown to cause autosomal dominant cone and cone-rod dystrophies (adCODs and adCORDs, respectively), while homozygous or compound heterozygous mutations cause autosomal recessively inherited Leber Congenital Amaurosis (LCA), the most severe form of inherited retinopathy, resulting in total blindness or greatly impaired vision at birth or in early infancy.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-RetGC-1 Antibody, clone 6D8.2 is validated for use in Western Blotting for the detection of GUCY2D.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant human RetGC-1/GUCY2D kinase domain fragmen.
EpitopeKinase domain.
Clone6D8.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 6D8.2 targets an epitope in the cytoplasmic kinase domain of human retinal guanylyl cyclase 1 (RetGC-1) encoded by the GUCY2D gene.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GUCY2D
  • CORD6
  • GUC1A4
  • GUC2D
  • RETGC
  • RETGC1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~120 kDa observed. 114.7/120.1 kDa (mature/pro-form) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Y79 human retinoblastoma cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected RetGC-1/GUCY2D in 10 µg of Y79 human retinoblastoma cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN1598 04055977353525