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16-455 Anti-Phosphoserine Antibody, clone 4A4, HRP Conjugate

16-455
10 blots  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWBMHRPMonoclonal Antibody
Description
Catalogue Number16-455
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Phosphoserine Antibody, clone 4A4, HRP Conjugate
Alternate Names
  • 4A4
  • anti-phosphoserine
  • phosphoserine antibody
  • pan-phosphoserine
Background InformationThe identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotrans-mission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that over 95% of all phosphorylation events occur on serine and threonine residues.
Product Information
FormatHRP
Presentation100 μL of protein G purified mouse IgG1 in PBS containing 0.05% Kathon® and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:4000 in 10 mL blocking buffer. Liquid.
Quality LevelMQ100
Applications
ApplicationAnti-Phosphoserine Antibody, clone 4A4, HRP Conjugate is an antibody against Phosphoserine for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenPhosphoserine conjugated to KLH.
Epitopephosphoserine
Clone4A4
HostMouse
IsotypeIgG1
Species Reactivity
  • All
Species Reactivity NoteRecognizes proteins containing phosphoserine residues, regardless of origin.
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein G Purified
Molecular WeightVaries
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size10 blots
Global Trade Item Number
Catalogue Number GTIN
16-455 04053252589614