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ST1643 Anti-PDK2 Mouse mAb (2G1)

This Anti-PDK2 Mouse mAb (2G1) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of PDK2.

Anti-PDK2 Mouse mAb (2G1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Pyruvate Dehydrogenase Kinase 2

ST1643
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
ST1643-100UGCN 100 μg
Description
OverviewRecognizes the ~40 kDa PDK2 protein in U-2 OS cells.
Catalogue NumberST1643
Brand Family Calbiochem®
SynonymsAnti-Pyruvate Dehydrogenase Kinase 2
Application Data
Detection of human PDK2 by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human PDK2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (0.8 µg/ml). Detection: colorimetric (DAB).
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlU-2 OS cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (0.8 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 187-276 human PDK2, expressed as a GST fusion protein
Clone2G1
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1643-100UGCN 04055977223781

Documentation

Anti-PDK2 Mouse mAb (2G1) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-November-2010 RFH
SynonymsAnti-Pyruvate Dehydrogenase Kinase 2
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (0.8 µg/ml)
Application Data
Detection of human PDK2 by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human PDK2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (0.8 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~40 kDa PDK2 protein.
BackgroundThe PDK2 protein inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 α subunit, thus contributing to the regulation of glucose metabolism.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 187-276 human PDK2, expressed as a GST fusion protein
Clone2G1
IsotypeIgG2a
Specieshuman
Positive controlU-2 OS cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review