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ST1508 Anti-PCK1 Mouse mAb (3E4)

This Anti-PCK1 Mouse mAb (3E4) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of PCK1.

Anti-PCK1 Mouse mAb (3E4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Phosphenolpyruvate Carboxykinase 1

ST1508
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
ST1508-100UGCN 100 μg
Description
OverviewRecognizes the ~70 kDa PCK1 protein in HepG2 cells.
Catalogue NumberST1508
Brand Family Calbiochem®
SynonymsAnti-Phosphenolpyruvate Carboxykinase 1
Application Data
Detection of human PCK1 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-PCK1 Mouse mAb (3E4) (Cat. No. ST1508) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human PCK1 by immunocytochemistry. Sample: HepG2 cells fixed in paraformaldehyde. Primary antibody: Anti-PCK1 Mouse mAb (3E4) (Cat. No. ST1508) (10 µg/ml). Detection: fluorescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlHepG2 cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunofluorescence
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant human PCK1 (aa 1-623), expressed as a GST fusion protein
Clone3E4
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1508-100UGCN 04055977224160

Documentation

Anti-PCK1 Mouse mAb (3E4) SDS

Title

Safety Data Sheet (SDS) 

Anti-PCK1 Mouse mAb (3E4) Certificates of Analysis

TitleLot Number
ST1508
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-November-2010 RFH
SynonymsAnti-Phosphenolpyruvate Carboxykinase 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human PCK1 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-PCK1 Mouse mAb (3E4) (Cat. No. ST1508) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human PCK1 by immunocytochemistry. Sample: HepG2 cells fixed in paraformaldehyde. Primary antibody: Anti-PCK1 Mouse mAb (3E4) (Cat. No. ST1508) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~70 kDa PCK1 protein.
BackgroundPCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency.
HostMouse
ImmunogenFull-length, recombinant human PCK1 (aa 1-623), expressed as a GST fusion protein
Clone3E4
IsotypeIgG₁
Specieshuman
Positive controlHepG2 cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review