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AB5164-50UL Anti-Muscarinic Acetylcholine Receptor m1 Antibody

Anti-Muscarinic Acetylcholine Receptor m1 Antibody is an antibody against Muscarinic Acetylcholine Receptor m1 for use in IH & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: CHRM1, M1, HM1, MGC30125 UniProt Number: P11229
AB5164-50UL
50 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RIHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5164-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Muscarinic Acetylcholine Receptor m1 Antibody
Product Information
FormatAffinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 60 μg of control antigen, molecular weight 40kDa (lyophilized powder). The stock solution of the antigen can be made up using 100 mL of PBS. The stock solution of the antigen can be made up using 200 μL of sterile deionized water. For positive control, in Western blot using 50 ng of protein per lane. For negative control, preincubate 3-5 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Quality LevelMQ100
Applications
ApplicationAnti-Muscarinic Acetylcholine Receptor m1 Antibody is an antibody against Muscarinic Acetylcholine Receptor m1 for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern blot: 1:200 (1-1.5 μg/mL) using ECL on rat brain membranes with proteinase inhibitors. M1 is approximately 60-80kDa in size, some preparations yield 125kDa dimers (photo).

Dilutions should be made using a carrier protein such as BSA (1-3%).

Immunohistochemistry: rat brain frozen sections. 1:50-1:200, internal epitope; use triton X-100 in blocking buffer only; dilute primary antibody in PBS or TBS with 0.5%-1% BSA or NGS only.



Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenGST fusion protein and part of i3 intercellular loop of human m1 muscarinic acetylcholine receptor (amino acids 227-353) (Accession P11229).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes a full length m1 protein. It has exhibited no cross reactivity with other muscarinic proteins tested so far.
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.
Gene Symbol
  • CHRM1
  • M1
  • HM1
  • MGC30125
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P11229 # The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
SIZE: 460 amino acids; 51421 Da
SUBUNIT: Interacts with GPRASP2.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P11229 ## Belongs to the G-protein coupled receptor 1 family.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Global Trade Item Number
Catalogue Number GTIN
AB5164-50UL 04053252506697