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NA26 Anti-MSH2 (Ab-1) Mouse mAb (GB12)

This Anti-MSH2 (Ab-1) Mouse mAb (GB12) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of MSH2 (Ab-1).

Anti-MSH2 (Ab-1) Mouse mAb (GB12) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Mismatch Repair Protein 2

NA26
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
NA26-100UGCN Plastic ampoule 100 μg
Description
OverviewRecognizes the ~100 kDa MSH2 protein in HCT116 and SW480 cells and colon tissue.
Catalogue NumberNA26
Brand Family Calbiochem®
SynonymsAnti-Mismatch Repair Protein 2
References
ReferencesBronner, C. E., et al. 1994. Nature 368, 258.
Papadopoulos, N., et al. 1994. Science 263, 1625.
Peltomäki, P. T. 1994. Annals of Medicine 26, 215.
Fishel, R., et al. 1993. Cell 75, 1027-1038, 1993.
Leach, F. S., et al. 1993. Cell 75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics 5, 279.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Negative controlLoVo cells
Positive controlHCT116 or SW480 cells or colon tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (5 µg/ml)
Immunoblotting (1 µg/ml)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (3 µg/ml, heat pre-treatment required)
Application CommentsFor paraffin sections, pre-treat with heat for 20 min. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenan N-terminal fragment of human MSH2
ImmunogenHuman
CloneGB12
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NA26-100UGCN 04055977227703

Documentation

Anti-MSH2 (Ab-1) Mouse mAb (GB12) SDS

Title

Safety Data Sheet (SDS) 

Anti-MSH2 (Ab-1) Mouse mAb (GB12) Certificates of Analysis

TitleLot Number
NA26

References

Reference overview
Bronner, C. E., et al. 1994. Nature 368, 258.
Papadopoulos, N., et al. 1994. Science 263, 1625.
Peltomäki, P. T. 1994. Annals of Medicine 26, 215.
Fishel, R., et al. 1993. Cell 75, 1027-1038, 1993.
Leach, F. S., et al. 1993. Cell 75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics 5, 279.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-September-2007 RFH
SynonymsAnti-Mismatch Repair Protein 2
ApplicationFrozen Sections (5 µg/ml)
Immunoblotting (1 µg/ml)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (3 µg/ml, heat pre-treatment required)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP20 mouse myeloma cells. Recognizes the ~100 kDa MSH2 protein.
BackgroundThe MSH2 gene is one of at least 4 genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma, while germline MSH2 mutations are responsible for ~50% of inherited, non-polyposis colorectal carcinoma (HNPCC). MSH2 encodes a protein of 934 amino acids.
HostMouse
Immunogen speciesHuman
Immunogenan N-terminal fragment of human MSH2
CloneGB12
IsotypeIgG₁
Specieshuman, not mouse
Positive controlHCT116 or SW480 cells or colon tissue
Negative controlLoVo cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsFor paraffin sections, pre-treat with heat for 20 min. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesBronner, C. E., et al. 1994. Nature 368, 258.
Papadopoulos, N., et al. 1994. Science 263, 1625.
Peltomäki, P. T. 1994. Annals of Medicine 26, 215.
Fishel, R., et al. 1993. Cell 75, 1027-1038, 1993.
Leach, F. S., et al. 1993. Cell 75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics 5, 279.