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IM51 Anti-MMP-2 (Ab-4) Mouse mAb (75-7F7)

Anti-MMP-2 (Ab-4) Mouse mAb (75-7F7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-72 kDa Gelatinase, Anti-Gelatinase A, Anti-Matrix Metalloproteinase 2

IM51
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, MMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
IM51-100UGCN Plastic ampoule 100 μg
Description
OverviewRecognizes the ~72 kDa latent and the ~66 kDa active forms of MMP-2.
Catalogue NumberIM51
Brand Family Calbiochem®
SynonymsAnti-72 kDa Gelatinase, Anti-Gelatinase A, Anti-Matrix Metalloproteinase 2
Application Data
Detection of human MMP-2 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts CCD-41SK cells. Primary antibody: Anti-MMP-2 (Ab-4) Mouse mAb (75-7F7) (Cat. No. IM51) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesCottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861.
Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91.
Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
Woessner, J. F., 1991. FASEB J. 5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Product Information
DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Negative controlMMP-9 protein (Cat. Nos. PF024 or PF038)
Positive controlMMP-2 protein (Cat. Nos. PF023 or PF037)
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91. Paraffin Sections Tsunezuka, Y., et al. 1996. Cancer Res., 56, 5678. Immunohistochemistry Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen Sections (see application references)
Immunoblotting (1 µg/ml, see application references)
Paraffin Sections (see application references)
Application CommentsThis antibody does not cross-react with human MMP-1, MMP-3, MMP-7, MMP-8, MMP-9 or MMP-13. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenhuman MMP-2 derived from conditioned medium of CCD-41SK skin fibroblasts
ImmunogenHuman
Clone75-7F7
HostMouse
IsotypeIgG₁
Species Reactivity
  • Bovine
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IM51-100UGCN 07790788053802

Documentation

Anti-MMP-2 (Ab-4) Mouse mAb (75-7F7) Certificates of Analysis

TitleLot Number
IM51

References

Reference overview
Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861.
Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91.
Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
Woessner, J. F., 1991. FASEB J. 5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-August-2007 RFH
SynonymsAnti-72 kDa Gelatinase, Anti-Gelatinase A, Anti-Matrix Metalloproteinase 2
ApplicationFrozen Sections (see application references)
Immunoblotting (1 µg/ml, see application references)
Paraffin Sections (see application references)
Application Data
Detection of human MMP-2 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts CCD-41SK cells. Primary antibody: Anti-MMP-2 (Ab-4) Mouse mAb (75-7F7) (Cat. No. IM51) (1 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~72 kDa latent and the ~66 kDa active forms of MMP-2.
BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-2 (Gelatinase A, 72 kDa gelatinase, type IV collagenase, TBE-1) is secreted as a 72 kDa zymogen which is proteolytically processed to the 66 kDa active form. MMP-2, along with its most closely related member of the MMP family, MMP-9, show substrate specificity toward type IV and V collagens, gelatin and elastin. Numerous studies have shown a correlation between collagenase expression and metastatic potential and suggest that it may be a useful marker for the diagnosis or prognosis of cancer.
HostMouse
Immunogen speciesHuman
Immunogenhuman MMP-2 derived from conditioned medium of CCD-41SK skin fibroblasts
Clone75-7F7
IsotypeIgG₁
Speciesbovine, human, mouse, not rat
Positive controlMMP-2 protein (Cat. Nos. PF023 or PF037)
Negative controlMMP-9 protein (Cat. Nos. PF024 or PF038)
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody does not cross-react with human MMP-1, MMP-3, MMP-7, MMP-8, MMP-9 or MMP-13. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861.
Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91.
Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
Woessner, J. F., 1991. FASEB J. 5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Application referencesImmunoblotting Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91. Paraffin Sections Tsunezuka, Y., et al. 1996. Cancer Res., 56, 5678. Immunohistochemistry Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69.