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IM35 Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5)

Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
IM35
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
IM35-100UGCN Plastic ampoule 100 μg
Description
OverviewRecognizes the ~55 kDa latent and the ~43 kDa active forms of MMP-1.
Catalogue NumberIM35
Brand Family Calbiochem®
Application Data
Detection of human MMP-1 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts CCD-41SK cells. Primary antibody: Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5) (Cat. No. IM35) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB 7, 1434.
Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1.
Woessner, J.F. 1991. FASEB 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1; 99-106.
Product Information
DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesParaffin Sections Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1. Frozen Sections Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen sections (see application references)
Immunoblotting (1 µg/ml)
Paraffin sections (2.5 µg/ml, pressure cooker pre-treatment required)
Application CommentsTo prepare conditioned medium for positive control, incubate HT-1080 cells for 2 h at 37°C in serum-free media containing 100 nM PMA. Collect and centrifuge medium; concentrate as necessary. Does not cross-react with bovine. Will not cross-react with human MMP-2, MMP-3, MMP-8, MMP-9, or MMP-13. For best results with paraffin sections, pre-treat with a pressure cooker; however, heat, saponin or trypsin can be used in place of a pressure cooker. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (VQGQNVLHGYPKDIYSSFG) corresponding to amino acids 332-350 of human MMP-1
ImmunogenHuman
Clone41-1E5
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IM35-100UGCN 04055977220568

Documentation

Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5) SDS

Title

Safety Data Sheet (SDS) 

Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5) Certificates of Analysis

TitleLot Number
IM35

References

Reference overview
Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB 7, 1434.
Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1.
Woessner, J.F. 1991. FASEB 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1; 99-106.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-October-2007 JSW
ApplicationFrozen sections (see application references)
Immunoblotting (1 µg/ml)
Paraffin sections (2.5 µg/ml, pressure cooker pre-treatment required)
Application Data
Detection of human MMP-1 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts CCD-41SK cells. Primary antibody: Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5) (Cat. No. IM35) (1 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~55 kDa latent and the ~43 kDa active forms of MMP-1.
BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-1 (interstitial collagenase, tissue collagenase, fibroblast collagenase) is secreted as a 57/52 kDa zymogen which is proteolytically processed to the 46/42 kDa active forms. This enzyme displays substrate specificity toward type I, II, III, VII, VIII and X collagens and gelatin. MMP-1 is thought to play an important role in pathophysiological degradation processes associated with conditions such as rheumatoid arthritis, osteoarthritis, and cancer cell invasion.
HostMouse
Immunogen speciesHuman
Immunogena synthetic peptide (VQGQNVLHGYPKDIYSSFG) corresponding to amino acids 332-350 of human MMP-1
Clone41-1E5
IsotypeIgG2a
Speciesnot bovine, human
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsTo prepare conditioned medium for positive control, incubate HT-1080 cells for 2 h at 37°C in serum-free media containing 100 nM PMA. Collect and centrifuge medium; concentrate as necessary. Does not cross-react with bovine. Will not cross-react with human MMP-2, MMP-3, MMP-8, MMP-9, or MMP-13. For best results with paraffin sections, pre-treat with a pressure cooker; however, heat, saponin or trypsin can be used in place of a pressure cooker. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB 7, 1434.
Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1.
Woessner, J.F. 1991. FASEB 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1; 99-106.
Application referencesParaffin Sections Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1. Frozen Sections Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69.