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MABE222 Anti-JMJD2A Antibody, clone 8B7.1, rabbit monoclonal

This Anti-JMJD2A Antibody, clone 8B7.1 is validated for use in Western Blotting, IHC for the detection of JMJD2A.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: KDM4A, JHDM3A, JMJD2, KIAA0677 UniProt Number: O75164
MABE222
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCRbPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE222
DescriptionAnti-JMJD2A Antibody, clone 8B7.1, rabbit monoclonal
Alternate Names
  • JMJD
  • Lysine-specific demethylase 4A
  • JmjC domain-containing histone demethylation protein 3A
  • Jumonji domain-containing protein 2A
Background InformationJumonji domain-containing protein 2A (JMJD2A), or KDM4A, is a ubiquitously expressed demethylase enzyme, which belongs to the JMJD2 family. Like other members of this family, JMJD2A contains multiple domains: two Tudor domains, two PHD-type zinc fingers, one JmjC domain, and one JmjN domain. Little is known about the function of most of these domains; however, they are believed to be involved in transcriptional regulation. JMJD2A functions as a histone demethylase with specific activity against trimethylated Histone H3 Lysine residues Lys-9 and Lys-36. It has no effect on any other lysine residues, or on mono- and dimethylated Lys-9 and Lys-36. The demethylation of these residues induces the production of succinate and formaldehyde. Studies have shown that JMJD2A recruits NCOR1 and histone deacetylases, causing the respective transcriptional repression of E2Fand ASCL2 responsive promoters.
Product Information
FormatPurified
Control
  • U2OS nuclear extract
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-JMJD2A Antibody, clone 8B7.1 is validated for use in Western Blotting, IHC for the detection of JMJD2A.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:100 dilution from a representative lot detected JMJD2A in human colorectal adenocarcinoma cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human JMJD2A.
Clone8B7.1
HostRabbit
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KDM4A
  • JHDM3A
  • JMJD2
  • KIAA0677
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~120 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in U2OS nuclear extract.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected JMJD2A in 10 µg of U2OS nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
MABE222 04053252698583