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CBL158 Anti-Integrin β2 [CD18] Antibody

CBL158
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, WB, IHC, FUNCMPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL158
ReplacesCBL514
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Integrin β2 [CD18] Antibody
Alternate Names
  • CD18
Product Information
FormatPurified
PresentationThe monoclonal is presented in phosphate buffered saline containing 15mM sodium azide, pH 7.4. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Quality LevelMQ100
Applications
ApplicationAnti-Integrin β2 [CD18] Antibody detects level of Integrin β2 [CD18] & has been published & validated for use in FC, WB, IH.
Key Applications
  • Flow Cytometry
  • Western Blotting
  • Immunohistochemistry
  • Affects Function
Application NotesImmunohistochemistry: Identification of CD18+ leucocytes on cryostat sections

Function Blocking: Studies of cell-cell and cell-matrix adhesion, this antibody will block cell-cell interaction

Flow Cytometry

Western Blot

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenLeucocytes from an LGL - type leukaemia
CloneMEM 48
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes the CD18 molecule, a 95 kDa integrin molecule (beta 2 integrin) that forms the common beta chain of the CD11/CD18 heterodimer integrins which are expressed on all leucocytes. The beta2 integrin is the ICAM 1 receptor

FUSION PARTNER:

Sp 2/0 - Ag 14 myeloma cell line
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe ITGB2 protein product is the integrin beta chain beta 2. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
Gene Symbol
  • ITGB2
  • MFI7
  • LAD
  • CD18
  • MF17
  • LCAMB
  • LFA-1
  • MAC-1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P05107 # Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha- D/beta-2 is a receptor for ICAM3 and VCAM1.
SIZE: 769 amino acids; 84782 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-2 associates with either alpha-L, alpha-M, alpha-X or alpha-D. Interacts with COPS5 and RANBP9.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
PTM: Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.DISEASE:SwissProt: P05107 # Defects in ITGB2 are the cause of leukocyte adhesion deficiency type I (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
SIMILARITY: SwissProt: P05107 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2o-8oC for up to 12 months from date of receipt. For long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
CBL158 04053252742859