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MAB1997-AF647 Anti-Integrin β1 Antibody, clone MB1.2 Antibody, Alexa Fluor® 647 Conjugate

Anti-Integrin 1, clone MB1.2, Alexa Fluor® 647 Conjugate, Cat. No. MAB1997-AF647, is a highly specific rat monoclonal antibody that targets Integrin 1 and has been tested in Flow Cytometry and Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Itgb1, Fnrb UniProt Number: P09055
MAB1997-AF647
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, ICCRAlexaFluor®647Monoclonal Antibody
Description
Catalogue NumberMAB1997-AF647
DescriptionAnti-Integrin β1 Antibody, clone MB1.2 Antibody, Alexa Fluor® 647 Conjugate
Alternate Names
  • Integrin beta-1
  • CD29
  • Fibronectin receptor subunit beta
  • GpIIa
  • VLA-1/2/3/4/5/6 subunit beta
Background InformationIntegrin beta-1 (UniProt P09055; also known as CD29, Fibronectin receptor subunit beta, GpIIa, VLA-1/2/3/4/5/6 subunit beta) is encoded by the Itgb1 (also known as Fnrb) gene (Gene ID 16412) in murine species. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.
References
Product Information
FormatAlexaFluor®647
PresentationPurified rat IgG2a Alexa Fluor® 647 conjugate in buffer containing PBS, 1.5% BSA with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Integrin 1, clone MB1.2, Alexa Fluor® 647 Conjugate, Cat. No. MAB1997-AF647, is a highly specific rat monoclonal antibody that targets Integrin 1 and has been tested in Flow Cytometry and Immunocytochemistry.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
Application NotesFlow Cytometry Analysis: 0.2 µL from a representative lot detected Integrin 1 on the surface of one million NIH/3T3 cells.

The unconjugated antibody (Cat. No. MAB1997) and FITC conjugate (Cat. No. FCMAB269F) are also available for Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blotting applications.
Biological Information
ImmunogenMouse Integrin beta-1 (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132).
CloneMB1.2
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityThe specificity of MB1.2 was determined by its binding to antigen, and the ability of antiserum against the carboxyl terminal of VLA-beta 1 subunit to deplete antigens for MB1.2 in sequential immunoprecipitation experiments. MB1.2 had no effect on cell adhesion to matrix proteins (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Itgb1
  • Fnrb
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~88 kDa (non-glycosylated) and ~130 kDa ( glycosylated).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in NIH/3T3 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Integrin-beta1 in NIH/3T3 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1997-AF647 04054839081439

Documentation

Anti-Integrin β1 Antibody, clone MB1.2 Antibody, Alexa Fluor® 647 Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-Integrin β1 Antibody, clone MB1.2 Antibody, Alexa Fluor® 647 Conjugate Certificates of Analysis

TitleLot Number
Anti-Integrin β1, clone MB1.2, -Q2766897 Q2766897
Anti-Integrin β1, clone MB1.2, Alexa Fluor® 647 Conjugate - 3197625 3197625
Anti-Integrin β1, clone MB1.2, Alexa Fluor® 647 Conjugate - 3732236 3732236
Anti-Integrin β1, clone MB1.2, Alexa Fluor® 647 Conjugate - 3923525 3923525
Anti-Integrin β1, clone MB1.2, Alexa Fluor® 647 Conjugate Monoclonal Antibody 3053774