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MABF2247-25UG Anti-ISG15 Antibody, clone 3C2

Anti-ISG15, clone 3C2, Cat. No. MABF2247, is an Armenian hamster monoclonal antibody that detects ISG15 and is tested for use in Western Blotting.

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25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABF2247-25UG
DescriptionAnti-ISG15 Antibody, clone 3C2
Alternate Names
  • Ubiquitin-like protein ISG15
  • Interferon-induced 15 kDa protein
  • Interferon-induced 17 kDa protein
  • IP17
  • Ubiquitin cross-reactive protein
Background InformationUbiquitin-like protein ISG15 (UniProt: Q64339; also known as Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, IP17, Ubiquitin cross-reactive protein) is encoded by the Isg15 (also known as G1p2, Ucrp) gene (Gene ID: 100038882) in murine species. ISG15 is a disulfide-linked homodimeric, interferon-induced protein that plays a key role in host antiviral response. It is reported to exist in three distinct states: free form, released into the extracellular space, and as conjugated to target proteins. It contains two ubiquitin-like domains (aa 2-76 and 77-155) that are essential for its efficient conjugation to cellular proteins. Ubiquitin-like 2 domain is essential for the first two steps allowing the linking of ISG15 to the E1 and E2 enzymes while Ubiquitin-like 1 domain is essential for the E3-mediated transfer of ISG15 from the E2 to the lysine residues of the target protein. It also requires its C-terminal LRLRGG motif to form intracellular conjugates. ISG15 plays key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGlylation is reported to involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes that catalyze the conjugation of ISG15 to a lysine residue in the target protein. Some of the target proteins of ISG15 include JAK1, ERK1, ERK2, STAT5A, TRIM25, and globin. The secreted form is also known to induce natural killer cell proliferation, act as a chemotactic factor for neutrophils, and act as a IFN -inducing cytokine. The secreted form acts through the integrin ITGAL/ITGB2 receptor to initiate activation of SRC family tyrosine kinases including LYN, HCK and FGR that leads to secretion of IFN and IL-10. S-nitrosylation of ISG15 can decrease its dimerization, thereby increasing the availability as well as the solubility of monomeric ISG15 for its conjugation to cellular proteins. (Ref.: Perng, Y-C., and Lenschow, DJ. (2018). Nat. Rev. Microbiol. 16(7); 423-439; Lenschow, DJ., et al. (2007). Proc. Natl. Acad. Sci, USA. 104(4); 1371-1376; Lenschow, DJ., et al. (2005). J. Virol. 79(22); 13974-13983).
References
Product Information
FormatPurified
PresentationPurified Armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-ISG15, clone 3C2, Cat. No. MABF2247, is an Armenian hamster monoclonal antibody that detects ISG15 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:250 dilution from a representative lot detected ISG15 in primary mouse embryonic fibroblasts infected with Chikungunya virus.

Western Blotting Analysis: A representative lot detected ISG15 in Western Blotting applications (Lenschow, D.J., et al. (2005). J Virol. 79(22):13974-83; Lenschow, D.J., et al. (2007). Proc Natl Acad Sci USA. 104(4):1371-6; Fan, J-B., et al. (2015). Sci Rep. 5:12704).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenHis-tagged full-length recombinant mouse Ubiquitin-like protein ISG15.
EpitopeUnknown
Clone3C2
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostArmenian Hamster
SpecificityClone 3C2 is an Armenian Hamster monoclonal antibody that detects Ubiquitin-like protein ISG15.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Isg15
Purification MethodProtein G purified
UniProt Number
Molecular Weight~16 kDa observed; 17.9 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity confirmation by isotyping test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be Armenian hamster IgG.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2247-25UG 04065270845375