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CP53 Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1)

This Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) is validated for use in Cell Inhibition Assays, FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of ICAM-1 (Ab-2).

Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CD54, Anti-Inter-Cellular Adhesion Molecule

CP53
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
CP53-100UGCN Plastic ampoule 100 μg
Description
OverviewRecognizes the ~85-115 kDa ICAM-1 protein in endothelial cells and tonsil tissue.
Catalogue NumberCP53
Brand Family Calbiochem®
SynonymsAnti-CD54, Anti-Inter-Cellular Adhesion Molecule
References
ReferencesTakami, S., et al. 1998. Circ. 97, 721.
Craig, A.G., et al. 1997. Infect. Immun. 65, 4580.
DeGraba, T.J. 1997. Neurology 49, S15.
Elangbam, C.S., et al. 1997. Vet. Pathol. 34, 61.
McCormick, C.J., et al. 1997. J. Clin. Invest. 15, 2521.
Acevedo, A., et al. 1993. Am. J. Pathol. 143, 774.
De Fougerolles, A.R., et al. 1993. J. Exp. Med. 177, 1187.
Fawcett, J., et al. 1992. Nature 360, 481.
Vazeux, R. et al. 1992. Nature 360, 485.
Boyd, A.W., et al. 1989. Blood 73, 1896.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Positive controlEndothelial cells or tonsil tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Boyd, A.W., et al. 1988. PNAS 85, 3095.
Key Applications Cell Inhibition Assays
Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Not Paraffin Sections
Application NotesCell Inhibition Assays (see comments)
Flow Cytometry (see comments)
Frozen Sections (1:50-1:100)
Immunoblotting (1:200-1:400)
Immunofluorescence (see comments)
Immunoprecipitation (10 µl/mg protein lysate)
Paraffin Sections (not recommended)
Application CommentsThis antibody will block aggregation of cell lines mediated by ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and aggregation of mixed T- and B-cell blasts. Will inhibit T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T cell mediated B-cell activation) is significantly inhibited. This antibody will also block elements of both effector arms of the immune system (cytotoxic cell function and Ig production). This antibody has been reported to work for immunofluorescence and flow cytometry studies. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenRaji cells
ImmunogenHuman
CloneW-CAM-1
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CP53-100UGCN 04055977221008

Documentation

Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) SDS

Title

Safety Data Sheet (SDS) 

Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) Certificates of Analysis

TitleLot Number
CP53

References

Reference overview
Takami, S., et al. 1998. Circ. 97, 721.
Craig, A.G., et al. 1997. Infect. Immun. 65, 4580.
DeGraba, T.J. 1997. Neurology 49, S15.
Elangbam, C.S., et al. 1997. Vet. Pathol. 34, 61.
McCormick, C.J., et al. 1997. J. Clin. Invest. 15, 2521.
Acevedo, A., et al. 1993. Am. J. Pathol. 143, 774.
De Fougerolles, A.R., et al. 1993. J. Exp. Med. 177, 1187.
Fawcett, J., et al. 1992. Nature 360, 481.
Vazeux, R. et al. 1992. Nature 360, 485.
Boyd, A.W., et al. 1989. Blood 73, 1896.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2007 RFH
SynonymsAnti-CD54, Anti-Inter-Cellular Adhesion Molecule
ApplicationCell Inhibition Assays (see comments)
Flow Cytometry (see comments)
Frozen Sections (1:50-1:100)
Immunoblotting (1:200-1:400)
Immunofluorescence (see comments)
Immunoprecipitation (10 µl/mg protein lysate)
Paraffin Sections (not recommended)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing Balb/c splenocytes with p3-NS-1/Ag4-1 (NS1) mouse myeloma cells (see application references). Recognizes the ~85-115 kDa ICAM-1 protein.
BackgroundCell adhesion molecules are membrane glycoproteins that play a major role in inflammation and neoplastic disease. Four primary groups of adhesion molecules have been described, which includes the integrin receptor family, the immunoglobulin superfamily, selectins, and cadherins. The intercellular adhesion molecules (ICAM-1, ICAM-2, and ICAM-3) belong to the immunoglobulin superfamily that are the counter receptors for the leukocyte integrin, lymphocyte function associated antigen 1 (LFA-1, CD11a/CD18). ICAM-1 (CD54) is a single chain glycoprotein with 7 potential N-linked glycosylation sites. It is expressed in an inducible dependent manner by endothelial and epithelial cells as well as fibroblasts, macrophages, lymphocytes, and leukocytes. ICAM-1 can mediate adhesion by binding to CD11b/CD18 (Mac-1) enhancing antigen specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected erythrocytes distinct from the LFA-1 binding site. Studies indicate that ICAM-1 may also be involved in the development of atherosclerotic plaques; increased expression of ICAM-1 has been observed in all types of atherosclerotic lesions except fibrous placques. It is thought that endothelial cell expression of ICAM-1 mediates the recruitment of leukocytes to the lesion as it is suggested by its role in inflammation.
HostMouse
Immunogen speciesHuman
ImmunogenRaji cells
CloneW-CAM-1
IsotypeIgG2b
Specieshuman
Positive controlEndothelial cells or tonsil tissue
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody will block aggregation of cell lines mediated by ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and aggregation of mixed T- and B-cell blasts. Will inhibit T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T cell mediated B-cell activation) is significantly inhibited. This antibody will also block elements of both effector arms of the immune system (cytotoxic cell function and Ig production). This antibody has been reported to work for immunofluorescence and flow cytometry studies. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Toxicity Standard Handling
ReferencesTakami, S., et al. 1998. Circ. 97, 721.
Craig, A.G., et al. 1997. Infect. Immun. 65, 4580.
DeGraba, T.J. 1997. Neurology 49, S15.
Elangbam, C.S., et al. 1997. Vet. Pathol. 34, 61.
McCormick, C.J., et al. 1997. J. Clin. Invest. 15, 2521.
Acevedo, A., et al. 1993. Am. J. Pathol. 143, 774.
De Fougerolles, A.R., et al. 1993. J. Exp. Med. 177, 1187.
Fawcett, J., et al. 1992. Nature 360, 481.
Vazeux, R. et al. 1992. Nature 360, 485.
Boyd, A.W., et al. 1989. Blood 73, 1896.
Application referencesOriginal Clone Boyd, A.W., et al. 1988. PNAS 85, 3095.