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CA1023 Anti-Hsp90α Mouse mAb (EMD-17D7)

This Anti-Hsp90α Mouse mAb (EMD-17D7) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Hsp90α.

Anti-Hsp90α Mouse mAb (EMD-17D7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Heat Shock Protein 90α

CA1023
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
CA1023-50UGCN Plastic ampoule 50 μg
Description
OverviewRecognizes the ~90 kDa Hsp90α protein in HeLa cells. Does not recognize Hsp90β.
Catalogue NumberCA1023
Brand Family Calbiochem®
SynonymsAnti-Heat Shock Protein 90α
Application Data
Detection of human Hsp90α by immunoblotting. Samples: Whole cell lysate (50 μg) from HeLa cells (lane A), Hsp90β, His•Tag®, Human, Recombinant (Cat. No. 385903) (100 ng, lane B), and Hsp90α, His•Tag®, Human, Recombinant (Cat. No. 385901) (100 ng, lane C). Primary antibody: Anti-Hsp90α Mouse mAb (EMD-17D7) (Cat. No. CA1023) (1 μg/ml). Detection: chemiluminescence.

Detection of human Hsp90α by immunoprecipitation and immunoblotting. Sample: Whole cell lysate (700 μg) from H-460 cells. Antibody for IP: Anti-Hsp90α Mouse mAb (EMD-17D7) (Cat. No. CA1023) (5 μg/700 μg protein). Conditions for immunoblotting: Sample: 10 μl of 50 μl Hsp90α IP (above). Primary antibody: Anti-Hsp90α Rabbit pAb (Cat. No. CA1016) (1:5000). Detection: chemiluminescence.
References
ReferencesScheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1297.
Nemoto, T., et al. 1997 J. Biol. Chem. 272, 26179.
Nemoto, T., et al. 1998. Biochem. J. 330, 989.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlH460 and HeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesELISA (see comments)
Immunoblotting (1 µg/ml)
Immunoprecipitation (5 µg/0.7 mg protein)
Application CommentsDetects native and recombinant Hsp90α. Does not recognize Hsp90β. This antibody will also work for ELISA, but concentration is assay dependent. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenHsp90α, His•Tag®, Human, Recombinant (Cat. No. 385901)
ImmunogenHuman
CloneEMD-17D7
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CA1023-50UGCN 04055977220780

Documentation

Anti-Hsp90α Mouse mAb (EMD-17D7) SDS

Title

Safety Data Sheet (SDS) 

Anti-Hsp90α Mouse mAb (EMD-17D7) Certificates of Analysis

TitleLot Number
CA1023

References

Reference overview
Scheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1297.
Nemoto, T., et al. 1997 J. Biol. Chem. 272, 26179.
Nemoto, T., et al. 1998. Biochem. J. 330, 989.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2007 RFH
SynonymsAnti-Heat Shock Protein 90α
ApplicationELISA (see comments)
Immunoblotting (1 µg/ml)
Immunoprecipitation (5 µg/0.7 mg protein)
Application Data
Detection of human Hsp90α by immunoblotting. Samples: Whole cell lysate (50 μg) from HeLa cells (lane A), Hsp90β, His•Tag®, Human, Recombinant (Cat. No. 385903) (100 ng, lane B), and Hsp90α, His•Tag®, Human, Recombinant (Cat. No. 385901) (100 ng, lane C). Primary antibody: Anti-Hsp90α Mouse mAb (EMD-17D7) (Cat. No. CA1023) (1 μg/ml). Detection: chemiluminescence.

Detection of human Hsp90α by immunoprecipitation and immunoblotting. Sample: Whole cell lysate (700 μg) from H-460 cells. Antibody for IP: Anti-Hsp90α Mouse mAb (EMD-17D7) (Cat. No. CA1023) (5 μg/700 μg protein). Conditions for immunoblotting: Sample: 10 μl of 50 μl Hsp90α IP (above). Primary antibody: Anti-Hsp90α Rabbit pAb (Cat. No. CA1016) (1:5000). Detection: chemiluminescence.
DescriptionProtein G PLUS/protein A purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90α protein.
BackgroundHsp90α is involved in signal transduction, cell cycle progression, and apoptosis. Hsp90α has been reported to interact with MMP-2 and promote its activation, which may, in turn, play a role in cancer cell invasiveness.
HostMouse
Immunogen speciesHuman
ImmunogenHsp90α, His•Tag®, Human, Recombinant (Cat. No. 385901)
CloneEMD-17D7
IsotypeIgG₁
Specieshuman
Positive controlH460 and HeLa cells
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDetects native and recombinant Hsp90α. Does not recognize Hsp90β. This antibody will also work for ELISA, but concentration is assay dependent. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Irritant
ReferencesScheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1297.
Nemoto, T., et al. 1997 J. Biol. Chem. 272, 26179.
Nemoto, T., et al. 1998. Biochem. J. 330, 989.