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MABS1998-25UG Anti-HPa3 Antibody, clone HIC3-2D12

Anti-HPa3, clone HIC3-2D12, Cat. No. MABS1998, is a mouse monoclonal antibody that detects human pancreatic non-beta endocrine cells and has been tested for use in Flow Cytometry and Immunohistochemistry (Paraffin).

Anti-HPa3 Antibody, clone HIC3-2D12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling UniProt Number: P48230
MABS1998-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1998-25UG
DescriptionAnti-HPa3 Antibody, clone HIC3-2D12
Alternate Names
  • Non-beta endocrine cells
Background InformationPancreas performs both the endocrine and the exocrine function. The bulk of pancreas is composed of exocrine cells that produce digestion enzymes which are released into ducts to be emptied into the duodenum. The endocrine pancreas is composed of islets of Langerhans that produce hormones. At least five distinct endocrine cell types have been identified in the endocrine pancreas, each producing a different hormone. Beta cells produce insulin, which is considered as an anabolic hormone and regulates blood glucose levels and is also involved in fat and protein synthesis. Alpha cells produce glucagon that acts when blood glucose levels are low. Ductal cells of pancreas form the epithelial lining of the branched tubes that deliver digestive enzymes into the duodenum. They also secrete bicarbonate to neutralize acidic contents coming in from stomach. Duct cells may contribute up to 10% of all pancreatic cells. Acinar cells function as a unit of the exocrine pancreas and they synthesize, store, and secrete digestive enzymes (lipase, alpha amylase, trypsin, and chymotrypsin), which under normal physiological conditions are activated only after reaching the duodenum. Premature activation of these enzymes within pancreatic acinar cells can lead to acute pancreatitis. Clone HIC-2D2 can be used to mark all non-beta endocrine cell types in human pancreatic tissue. (Ref.: Dorrell, C., et al. (2008). Stem Cell Research (2008). 1(3); 183-194; Grapin-Botton, A (2005). Int. J. Biochem. Cell Biol. 37(3); 504-510; Leung, PS (2006). Int. J. Biochem. Cell Biol. 38(7); 1024-1030).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Applications
ApplicationAnti-HPa3, clone HIC3-2D12, Cat. No. MABS1998, is a mouse monoclonal antibody that detects human pancreatic non-beta endocrine cells and has been tested for use in Flow Cytometry and Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: A representative lot detected HPa3 in Flow Cytometry applications (Dorrell, C., et. al. (2008). Stem Cell Res. 1(3):183-94; Bramswig, N.C., et. al. (2013). J Clin Invest. 123(3):1275-84; Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
Biological Information
ImmunogenHuman pancreatic islets enriched in non-beta endocine cells.
CloneHIC3-2D12
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone HIC3-2D12 detects human pancreatic non-beta endocrine cells.
IsotypeIgMκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human pancreatic tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected HPa3 in human pancreatic tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1998-25UG 04054839507090