Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

IF03L Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse mAb (G-A-5)

Anti-Glial Fibrillary Acidic Protein, mouse monoclonal, clone G-A-5, recognizes GFAP in astrocytes, astrocytomas, glial cells & gliomas. It is validated for use in WB, IF, IP, IHC (frozen & paraffin).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-GFAP

IF03L
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Ch, H, Porcine, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
IF03L-100UGCN Plastic ampoule 100 μg
Description
OverviewRecognizes GFAP in astrocytes, astrocytomas, glial cells, gliomas, and other glial cell-derived tumors.
Catalogue NumberIF03L
Brand Family Calbiochem®
SynonymsAnti-GFAP
References
ReferencesDroese, M., et al. 1984. Acta. Cytol. 28, 368.
Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
Geisler, N. and Weber, K. 1983. EMBO J. 11, 2059.
Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
Osborn, M. and Weber, K. 1982. Cell 31, 303.
Product Information
FormLyophilized
FormulationLyophilized from 20 mM NH₄HCO₃, 100 µg BSA.
Negative controlEpithelial cells
Positive controlGlial cells (such as U343/333MG)
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting, Paraffin Sections Sennlaub, F., et al. 2003. Circulation 108, 198. Original Clone Debus, E., et al. 1983. Differentiation 25, 193. Immunofluorescence Debus, E., et al. 1983. Differentiation 25, 193.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (see application references)
Paraffin Sections (see application references)
Frozen Sections (see comments)
Immunofluorescence (see application references)
Immunoprecipitation (see comments)
Application CommentsStains intermediate filaments in astrocytes, glial cells, gliomas, and other glial cell-derived tumors. This antibody has also been reported to work for frozen sections and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenGFAP
ImmunogenPorcine
CloneG-A-5
HostMouse
IsotypeIgG₁
Species Reactivity
  • Chicken
  • Human
  • Porcine
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsResuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product; avoid repetitive freezing and thawing.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IF03L-100UGCN 04055977228120

Documentation

Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse mAb (G-A-5) Certificates of Analysis

TitleLot Number
IF03L

References

Reference overview
Droese, M., et al. 1984. Acta. Cytol. 28, 368.
Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
Geisler, N. and Weber, K. 1983. EMBO J. 11, 2059.
Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
Osborn, M. and Weber, K. 1982. Cell 31, 303.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-August-2007 RFH
SynonymsAnti-GFAP
ApplicationImmunoblotting (see application references)
Paraffin Sections (see application references)
Frozen Sections (see comments)
Immunofluorescence (see application references)
Immunoprecipitation (see comments)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with PAI mouse myeloma cells (see application references). Recognizes the glial fibrillary acidic protein (GFAP).
BackgroundIntermediate (10 nm) filaments are present in, and provide information concerning the origin of, most vertebrate cells. At present, five major cell types can be distinguished by their intermediate filament components. These include epithelial cells (cytokeratins), neurons (neurofilaments), glial cells (glial fibrillary acidic protein or GFA or GFAP), skeletal, visceral, and certain vascular smooth muscle cells (desmin) and various nonepithelial cells, including cells of mesenchymal origin (vimentin). GFAP and vimentin are coexpressed in at least some astrocytes. Anti-GFAP (Ab-1) identifies the GFAP subclass of intermediate filaments in human tissues.
HostMouse
Immunogen speciesPorcine
ImmunogenGFAP
CloneG-A-5
IsotypeIgG₁
Specieschicken, human, porcine, rat
Positive controlGlial cells (such as U343/333MG)
Negative controlEpithelial cells
FormLyophilized
FormulationLyophilized from 20 mM NH₄HCO₃, 100 µg BSA.
PreservativeNone
CommentsStains intermediate filaments in astrocytes, glial cells, gliomas, and other glial cell-derived tumors. This antibody has also been reported to work for frozen sections and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsResuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product; avoid repetitive freezing and thawing.
Toxicity Standard Handling
ReferencesDroese, M., et al. 1984. Acta. Cytol. 28, 368.
Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
Geisler, N. and Weber, K. 1983. EMBO J. 11, 2059.
Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
Osborn, M. and Weber, K. 1982. Cell 31, 303.
Application referencesImmunoblotting, Paraffin Sections Sennlaub, F., et al. 2003. Circulation 108, 198. Original Clone Debus, E., et al. 1983. Differentiation 25, 193. Immunofluorescence Debus, E., et al. 1983. Differentiation 25, 193.