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MABT418 Anti-GPVI Antibody, clone 8A2, Detection Antibody

Anti-GPVI Antibody, clone 8A2, Detection Antibody is an antibody against GPVI for use in western blotting & ELISA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: GP6 UniProt Number: Q9HCN6
MABT418
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ELISARPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT418
ReplacesMABT473
DescriptionAnti-GPVI Antibody, clone 8A2, Detection Antibody
Alternate Names
  • Platelet glycoprotein VI
  • GPVI
  • Glycoprotein 6
Background InformationGlycoprotein VI (GPVI), or glycoprotein 6 (GP6), is a member of the immunoglobulin gene superfamily, closely related to Fc receptor gamma chain (FcRgamma) and natural killer receptors. GPVI is a collagen receptor involved in collagen-induced platelet adhesion and activation, and may contribute to arterial and venous thrombus formation. GPVI is a membrane glycoprotein that plays a crucial role in platelet procoagulant activity and subsequent thrombin and fibrin formation. GPVI is expressed in megakaryocytes and platelets. The signaling pathway of GPVI involves the FcRgamma, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. It has been shown that bleeding disorders such as Bleeding Disorder Platelet-Type 11 (BDPLT11), a mild to moderate bleeding disorder caused by defective platelet activation and aggregation in response to collagen, is caused by defects in GPVI.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GPVI Antibody, clone 8A2, Detection Antibody is an antibody against GPVI for use in western blotting & ELISA.
Key Applications
  • Western Blotting
  • ELISA
Application NotesELISA Assay: A representative lot from an independent laboratory detected recombinant GPVI in a bead-based sandwich immunoassay (Bigalke, B., et al. (2011). Clin Chem. 57(6):898-904.). This antibody should be used in conjunction with Cat. No. MABT406, Anti-GPVI, clone 8E9, Capture Antibody, as an ELISA detection and capture antibody pair.
Biological Information
ImmunogenRecombinant protein corresponding to human GPVI.
Clone8A2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GP6
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~50 kDa observed. The calculated molecular weight of this protein is 37 kDa. However, a recombinant protein was observed at 50-55 kDa in Western Blotting.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in recombinant human GPVI glycoprotein.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected recombinant human GPVI glycoprotein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT418 04053252955464

Documentation

Anti-GPVI Antibody, clone 8A2, Detection Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-GPVI Antibody, clone 8A2, Detection Antibody Certificates of Analysis

TitleLot Number
Anti-GPVI, clone 8A2, Detection Antibody - Q2383909 Q2383909
Anti-GPVI, clone 8A2, Detection Antibody - 3249928 3249928

References

Reference overviewPub Med ID
Sandwich immunoassay for soluble glycoprotein VI in patients with symptomatic coronary artery disease.
Bigalke, Boris, et al.
Clin. Chem., 57: 898-904 (2011)  2011

Show Abstract 		21474641 21474641