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AP1140 Anti-GBA Mouse mAb (2E2)

Anti-GBA, mouse monoclonal, clone 2E2, recognizes the ~60 kDa GBA in MCF-7 and HeLa cells and human breast cancer tissue. It is validated for use in ELISA, WB, ICC, and IHC on paraffin sections.

Anti-GBA Mouse mAb (2E2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Glucosidase β, Acid

AP1140
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
AP1140-100UGCN 100 μg
Description
OverviewRecognizes the ~60 kDa GBA protein in MCF-7 and HeLa cells and human breast cancer tissue.
Catalogue NumberAP1140
Brand Family Calbiochem®
SynonymsAnti-Glucosidase β, Acid
Application Data
Detection of human GBA by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human GBA by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (10 µg/ml). Detection: fluorescence.

Detection of human GBA by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human breast cancer tissue. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (3 µg/ml). Detection: colorimetric (DAB).
References
ReferencesCampeau, P.M., et al. 2009. Blood 114, 3181.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T
Positive controlMCF-7 cells, HeLa cells, Human breast cancer tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 146-236 human GBA, expressed as a GST fusion protein
Clone2E2
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1140-100UGCN 04055977227857

Documentation

Anti-GBA Mouse mAb (2E2) SDS

Title

Safety Data Sheet (SDS) 

Anti-GBA Mouse mAb (2E2) Certificates of Analysis

TitleLot Number
AP1140

References

Reference overview
Campeau, P.M., et al. 2009. Blood 114, 3181.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-October-2010 RFH
SynonymsAnti-Glucosidase β, Acid
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human GBA by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human GBA by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (10 µg/ml). Detection: fluorescence.

Detection of human GBA by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human breast cancer tissue. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (3 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~60 kDa GBA protein.
BackgroundAcid beta-glucosidase (GBA) is a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. It catalyzes the breakdown of the glycolipid glucosylceramide to ceramide and glucose. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 146-236 human GBA, expressed as a GST fusion protein
Clone2E2
IsotypeIgG2a
Specieshuman
Positive controlMCF-7 cells, HeLa cells, Human breast cancer tissue
Negative control293T
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesCampeau, P.M., et al. 2009. Blood 114, 3181.