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AB2302-25UG Anti-GAPDH

Anti-GAPDH Antibody detects level of GAPDH & has been published & validated for use in WB.

AB2302-25UG
25 μg  
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Description
Catalogue NumberAB2302-25UG
DescriptionAnti-GAPDH
Alternate Names
  • glyceraldehyde 3-phosphate dehydrogenase
  • aging-associated gene 9 protein
  • glyceraldehyde-3-phosphate dehydrogenase
Background InformationGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
References
Product Information
FormatAffinity Purified
Control
  • HEK293 cell lysate
PresentationPurified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GAPDH Antibody detects level of GAPDH & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern Blot (SNAP ID) Analysis: 0.125 µg/ml from a previous lot detected GAPDH on 10 µg of NIH/3T3 and PC12 cell lysates.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the WARS interacting region of human GAPDH.
EpitopeWARS interacting region
ConcentrationPlease refer to lot specific datasheet.
HostChicken
SpecificityThis antibody recognizes GAPDH.
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Horse
  • Canine
Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Predicted to react with equine and canine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq].
Gene Symbol
  • GAPDH
  • G3PDH
  • GAPD
  • G3PD
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.

SIZE: 335 amino acids; 36053 Da

SUBUNIT: Homotetramer. Binds PRKCI.

SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.

SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Molecular Weight~ 38 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HEK293 and PC12 cell lysate.

Western Blot Analysis: 0.0625 µg/ml of this antibody detected GAPDH on 10 µg of HEK293 and PC12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB2302-25UG 04054839342622

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