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MAB341 Anti-GABA A Receptor β 2,3 Chain Antibody, clone BD17

MAB341
100 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, H, M, R, Mk, FeIHC, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB341
ReplacesAB5952
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-GABA A Receptor β 2,3 Chain Antibody, clone BD17
Alternate Names
  • GABA(A) receptor subunit beta-2
  • gamma-aminobutyric acid (GABA) A receptor, beta 2
  • gamma-aminobutyric acid A receptor, beta 2
Background InformationGamma-aminobutyric acid type A (GABAA) receptors are members of the neurotransmitter ligand-gated ion channels that mediate neuronal inhibition on binding GABA. The effects of GABA on GABAA receptors are modulated by a range of therapeutically important drugs, including barbiturates, anaesthetics and benzodiazepines.
References
Product Information
FormatPurified
Control
  • HepG2 cell lysate
PresentationPurified mouse monoclonal IgG1 liquid in buffer containing 10 mM potassium phosphate buffer pH 7.4 with 150 mM NaCl
Quality LevelMQ100
Applications
ApplicationDetect GABA A Receptor β 2 using this Anti-GABA A Receptor β 2, 3 Chain Antibody, clone BD17 validated for use in IH, IP & WB.
Key Applications
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry:
A 10-20 μg/mL concetration of a previous lot was used in IH.

Immunoprecipitation:
A previous lot of this antibody was used in IP.

Western blot:
5-20 µg/mL. Recognizes a 55 kDa band corresponding to the beta chain of the GABAA receptor.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified GABA/benzodiazepine receptor from bovine cortex
EpitopeExtracellular domain of the beta2 & beta3 subunits of the GABAA receptor
CloneBD17
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with the β-chain of the GABAA receptor.
IsotypeIgG1
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
  • Monkey
  • Feline
Species Reactivity NoteExpected to react with human and rat based on sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes GABA A receptor, beta 2 subunit. It is mapped to chromosome 5q34 in a cluster comprised of genes encoding alpha 1 and gamma 2 subunits of the GABA A receptor. Alternative splicing of this gene generates 2 transcript variants, differing by a 114 bp insertion.
Gene Symbol
  • GABRB2
  • GABA
  • MGC119388
  • MGC119389
  • MGC119386
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P47870 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
SIZE: 474 amino acids; 54606 Da
SUBUNIT: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Binds UBQLN1.
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P47870 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family
Molecular Weight55 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot on mouse brain membrane lysates.

Western Blot:
1:500 dilution of this antibody detected GABA A Receptor β 2,3 Chain on 10 μg of mouse brain membrane lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB341 04053252397684

Documentation

Anti-GABA A Receptor β 2,3 Chain Antibody, clone BD17 SDS

Title

Safety Data Sheet (SDS) 

Anti-GABA A Receptor β 2,3 Chain Antibody, clone BD17 Certificates of Analysis

TitleLot Number
Anti-GABA A Receptor 2,3 Chain, extracellular domain of the 2 3 subunits of the GABA A receptor, clone BD17 - 1995590 1995590
Anti-GABA A Receptor 2,3 Chain, extracellular domain of the 2 3 subunits of the GABA A receptor, clone BD17 - LV1583051 LV1583051
Anti-GABA A Receptor 2,3 Chain, extracellular domain of the 2 3 subunits of the GABA A receptor, clone BD17 - LV1671295 LV1671295
Anti-GABA A Receptor 2,3 Chain, extracellular domain of the 2 3 subunits of the GABA A receptor, clone BD17 - LV1718393 LV1718393
Anti-GABA A Receptor 2,3 Chain, extracellular domain of the 2 3 subunits of the GABA A receptor, clone BD17 - LV1777813 LV1777813
Anti-GABA A Receptor 2,3 Chain, extracellular domain of the 2 3 subunits of the GABA A receptor, clone BD17 - LV1838660 LV1838660
Anti-GABA A Receptor 2,3 Chain, extracellular domain of the 2 & 3 subunits of the GABA A receptor, clone BD17 - 2279232 2279232
Anti-GABA A Receptor #946; 2,3 Chain, extracellular domain of the #946;2 #946;3 subunits of the GABA A receptor, clone BD17 - 2140036 2140036
Anti-GABA A Receptor #946; 2,3 Chain, extracellular domain of the #946;2 #946;3 subunits of the GABA A receptor, clone BD17 - 1940523 1940523
Anti-GABA A Receptor Beta 2,3 Chain, extracellular domain of the Beta2 Beta3 subunits of the GABA A receptor, clone BD17 - LV1462885 LV1462885

References

Reference overviewApplicationSpeciesPub Med ID
Altered neuronal excitability underlies impaired hippocampal function in an animal model of psychosis.
Grüter, T; Wiescholleck, V; Dubovyk, V; Aliane, V; Manahan-Vaughan, D
Frontiers in behavioral neuroscience  9  117  2015

Show Abstract
26042007 26042007
GABAA receptor activity shapes the formation of inhibitory synapses between developing medium spiny neurons.
Arama, J; Abitbol, K; Goffin, D; Fuchs, C; Sihra, TS; Thomson, AM; Jovanovic, JN
Frontiers in cellular neuroscience  9  290  2015

Show Abstract
26300728 26300728
Transmitter inputs to different motoneuron subgroups in the oculomotor and trochlear nucleus in monkey.
Zeeh, C; Mustari, MJ; Hess, BJ; Horn, AK
Frontiers in neuroanatomy  9  95  2015

Show Abstract
26257611 26257611
Human α1β3γ2L gamma-aminobutyric acid type A receptors: High-level production and purification in a functional state.
Dostalova, Z; Zhou, X; Liu, A; Zhang, X; Zhang, Y; Desai, R; Forman, SA; Miller, KW
Protein science : a publication of the Protein Society  23  157-66  2014

Show Abstract
24288268 24288268
Reduced GABAergic inhibition in the basolateral amygdala and the development of anxiety-like behaviors after mild traumatic brain injury.
Almeida-Suhett, CP; Prager, EM; Pidoplichko, V; Figueiredo, TH; Marini, AM; Li, Z; Eiden, LE; Braga, MF
PloS one  9  e102627  2014

Show Abstract
25047645 25047645
Rebound potentiation of inhibition in juvenile visual cortex requires vision-induced BDNF expression.
Gao, M; Maynard, KR; Chokshi, V; Song, L; Jacobs, C; Wang, H; Tran, T; Martinowich, K; Lee, HK
The Journal of neuroscience : the official journal of the Society for Neuroscience  34  10770-9  2014

Show Abstract
25100608 25100608
A novel GABRG2 mutation, p.R136*, in a family with GEFS+ and extended phenotypes.
Johnston, AJ; Kang, JQ; Shen, W; Pickrell, WO; Cushion, TD; Davies, JS; Baer, K; Mullins, JG; Hammond, CL; Chung, SK; Thomas, RH; White, C; Smith, PE; Macdonald, RL; Rees, MI
Neurobiology of disease  64  131-41  2014

Show Abstract
24407264 24407264
TNF-α Downregulates Inhibitory Neurotransmission through Protein Phosphatase 1-Dependent Trafficking of GABAA Receptors.
Pribiag, Horia and Stellwagen, David
J. Neurosci., 33: 15879-93 (2013)  2013

Show Abstract
24089494 24089494
Synaptic abnormalities in the infralimbic cortex of a model of congenital depression.
Seese, RR; Chen, LY; Cox, CD; Schulz, D; Babayan, AH; Bunney, WE; Henn, FA; Gall, CM; Lynch, G
The Journal of neuroscience : the official journal of the Society for Neuroscience  33  13441-8  2013

Show Abstract
23946402 23946402
GABA(A) receptors can initiate the formation of functional inhibitory GABAergic synapses.
Fuchs, C; Abitbol, K; Burden, JJ; Mercer, A; Brown, L; Iball, J; Anne Stephenson, F; Thomson, AM; Jovanovic, JN
The European journal of neuroscience  38  3146-58  2013

Show Abstract
23909897 23909897