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PC154 Anti-Excitatory Amino Acid Transporter (GLT-1) Rabbit pAb

Anti-Excitatory Amino Acid Transporter (GLT-1), rabbit polyclonal recognizes the ~75 kDa and the ~50 kDa forms of GLT-1. It is validated for use in Western blotting and immunoprecipitation.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC154
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PC154-100ULCN Plastic ampoule 100 ul
Description
OverviewRecognizes the ~75 kDa and the ~50 kDa forms of GLT-1.
Catalogue NumberPC154
Brand Family Calbiochem®
References
ReferencesArriza, J.L., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 4155.
Nagao, S., et al. 1997. Neuroscience 78, 929.
Rothstein, J.D., et al. 1995. Ann. Neurol. 14, 5559.
Arriza, J.L., et al. 1994. J. Neurosci. 14, 5559.
Rothstein, J.D., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 6591.
Kanai, Y., et al. 1992. Nature 360, 467.
Pines, G., et al. 1992. Nature 360, 464.
Rothstein, J.D., et al. 1992. N. Engl J. Med. 326, 1464.
Storck T., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 10955.
Product Information
FormLiquid
FormulationSerum diluted in PBS.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:1000)
Immunoprecipitation (see comments)
Application CommentsThis antibody may also cross react nonspecifically with a ~102 kDa protein not believed to be related to GLT-1. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (NGKSADCSVEEEPWKREK) corresponding to amino acids at the C-terminus of rat GLT-1
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC154-100ULCN 04055977209488

Documentation

Anti-Excitatory Amino Acid Transporter (GLT-1) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Excitatory Amino Acid Transporter (GLT-1) Rabbit pAb Certificates of Analysis

TitleLot Number
PC154

References

Reference overview
Arriza, J.L., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 4155.
Nagao, S., et al. 1997. Neuroscience 78, 929.
Rothstein, J.D., et al. 1995. Ann. Neurol. 14, 5559.
Arriza, J.L., et al. 1994. J. Neurosci. 14, 5559.
Rothstein, J.D., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 6591.
Kanai, Y., et al. 1992. Nature 360, 467.
Pines, G., et al. 1992. Nature 360, 464.
Rothstein, J.D., et al. 1992. N. Engl J. Med. 326, 1464.
Storck T., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 10955.

Brochure

Title
Pathways and Biomarkers of Glutamatergic Synapse Flyer
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-October-2007 RFH
ApplicationImmunoblotting (1:1000)
Immunoprecipitation (see comments)
DescriptionRabbit polyclonal antibody supplied as diluted serum. Recognizes the ~75 kDa and the ~50 kDa forms of GLT-1.
BackgroundPrecise regulation of excitatory amino acids (EAA) such as glutamate is essential, as glutamate is not only the major excitatory neurotransmitter, but also a potent excitotoxin. Defects in functional glutamate transport have been identified in the amyotrophic lateral sclerosis (ALS) brain and spinal cord, suggesting that defective glutamate transport may contribute to motor neuron degeneration. Three glutamate transporter subtypes have been identified in mammals, which include GLAST, GLT-1 and EAAC1. More recently, the homologous proteins EAAT1, EAAT2, and EAAT3 have been characterized in humans, respectively. EAAT4 and EAAT5 transporters have been localized to Purkinje cell synapses, and retina, respectively. The selective loss of glutamate transport in ALS appears to be specific for the GLT-1 (EAAT2) transporter subtyp. While GLT-1, GLAST and EAAC1 share approximately 50% sequence homology, the sequence used as the immunogen is unique to GLT-1.
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide (NGKSADCSVEEEPWKREK) corresponding to amino acids at the C-terminus of rat GLT-1
IsotypeIgG
Speciesrat
FormLiquid
FormulationSerum diluted in PBS.
Preservative≤0.1% sodium azide
CommentsThis antibody may also cross react nonspecifically with a ~102 kDa protein not believed to be related to GLT-1. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesArriza, J.L., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 4155.
Nagao, S., et al. 1997. Neuroscience 78, 929.
Rothstein, J.D., et al. 1995. Ann. Neurol. 14, 5559.
Arriza, J.L., et al. 1994. J. Neurosci. 14, 5559.
Rothstein, J.D., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 6591.
Kanai, Y., et al. 1992. Nature 360, 467.
Pines, G., et al. 1992. Nature 360, 464.
Rothstein, J.D., et al. 1992. N. Engl J. Med. 326, 1464.
Storck T., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 10955.