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MABC1800-100UG Anti-EZHIP Antibody, clone 1G11

Anti-EZHIP, clone 1G11, Cat. No. MABC1800, is a mouse monoclonal antibody that detects EZH inhibitory protein (EZHIP) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

MABC1800-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABC1800-100UG
DescriptionAnti-EZHIP Antibody, clone 1G11
Alternate Names
  • EZH inhibitory protein
Background InformationEZH inhibitory protein (UniProt: Q86X51; also known as EZHIP) is encoded by the EZHIP (also known as CXorf67) gene (Gene ID: 340602) in human. EZHIP is expressed predominantly in the gonads. In testis, it is detected in male germ cells inside the seminiferous tubules, especially in spermatogonia and round spermatids. In the ovary, it is expressed in primordial follicles and oocytes but not the external follicle cells. It is shown to inhibit PRC2/EED-EZH1 and PRC2/EED-EZH2 complex function by inhibiting EZH1/EZH2 methyltransferase activity, thereby causing down-regulation of histone H3 trimethylation on lysine 27 (H3K27me3). It inhibits H3K27me3 deposition during spermatogenesis and oogenesis. Its inhibitory effect on methyltransferase activity is attributed to its ability to limit the stimulatory effect of cofactors such as AEBP2 and JARID2. Amino acid sequence 403-423 is reported to be essential and sufficient for inhibition of PRC2/EED-EZH1 and PRC2/EED-EZH2 complex activity and the sequence 401-409 is sufficient for its interaction with EZH2. (Ref.: Ragazzini, R., et al. (2019). Nat. Commun. 10(1); 3858; Jain, SU., et al. (2019). Nat. Commun. 10(1); Article 2146).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-EZHIP, clone 1G11, Cat. No. MABC1800, is a mouse monoclonal antibody that detects EZH inhibitory protein (EZHIP) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunoprecipitation
Application NotesTested Applications

Immunofluorescence Analysis: A 1:1500 dilution from a representative lot detected EZHIP in Daoy cells.

Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated EZHIP in Daoy cell lysate.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 145 amino acids from the N-terminal half of human EZH inhibitory protein (EZHIP).
EpitopeN-terminal half
Clone1G11
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1G11 is a mouse monoclonal antibody that detects EZH inhibitory protein. It targets an epitope within the N-terminal half.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • EZHIP
Purification MethodProtein G purified
UniProt Number
Molecular Weight~60 kDa observed; 51.89 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Daoy cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EZHIP in Daoy cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1800-100UG 04065267327877