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DR1093 Anti-ETV6 Mouse mAb (3B10)

This Anti-ETV6 Mouse mAb (3B10) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of ETV6.

Anti-ETV6 Mouse mAb (3B10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ETS Variant 6

DR1093
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
DR1093-100UGCN 100 μg
Description
OverviewRecognizes the ~55-60 kDa ETV6 protein in Jurkat cells and human placenta tissue.
Catalogue NumberDR1093
Brand Family Calbiochem®
SynonymsAnti-ETS Variant 6
Application Data
Detection of human EVT6 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (1-5 µg/ml). Detection: chemilimunescence.

Detection of human EVT6 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (3 µg/ml). Detection: colorimetric (DAB).
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlJurkat cells, Human placenta tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human ETV6 (aa 1-453), expressed as a GST fusion protein
Clone3B10
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1093-100UGCN 04055977226126

Documentation

Anti-ETV6 Mouse mAb (3B10) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-November-2010 RFH
SynonymsAnti-ETS Variant 6
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human EVT6 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (1-5 µg/ml). Detection: chemilimunescence.

Detection of human EVT6 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (3 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~55-60 kDa ETV6 protein.
BackgroundETV6 contains two functional domains: a N-terminal pointed (PNT) domain that is involved in protein-protein interactions with itself and other proteins, and a C-terminal DNA-binding domain. Gene knockout studies in mice suggest that this protein is required for hematopoiesis and maintenance of the developing vascular network. This gene is known to be involved in a large number of chromosomal rearrangements associated with leukemia and congenital fibrosarcoma.
HostMouse
ImmunogenFull-length, recombinant, human ETV6 (aa 1-453), expressed as a GST fusion protein
Clone3B10
IsotypeIgG₁
Specieshuman
Positive controlJurkat cells, Human placenta tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review