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GR15L Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, IP, and neutralization studies.

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Epidermal Growth Factor Receptor

GR15L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
GR15L-100UGCN Plastic ampoule 100 μg
Description
OverviewRecognizes the ~170 kDa EGF receptor protein in A431 cells.
Catalogue NumberGR15L
Brand Family Calbiochem®
SynonymsAnti-Epidermal Growth Factor Receptor
Application Data
Detection of human EGFR by immunocytochemistry. Sample: A431 cells fixed with methanol. Primary Antibody: Anti-Epidermal Growth Factor Receptor (Ab-5) Mouse mAb (Cat. No. GR15L) (1 µg/ml). Detection: fluorescence.
References
ReferencesHunter T., 1984. Nature 311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
Product Information
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
Negative controlHL-60 cells
Positive controlA-431 cells
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesNeutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511.
Key Applications Flow Cytometry
Immunofluorescence
Immunoprecipitation
Neutralization Studies
Application NotesFlow Cytometry (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Neutralization Studies (1-2 µg/ml; see application references)
Application CommentsStains A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. This antibody does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpartially purified, human EGFR from A431 cells
ImmunogenHuman
CloneEGFR.1
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GR15L-100UGCN 04055977228250

Documentation

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1) SDS

Title

Safety Data Sheet (SDS) 

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1) Certificates of Analysis

TitleLot Number
GR15L

References

Reference overview
Hunter T., 1984. Nature 311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-August-2007 RFH
SynonymsAnti-Epidermal Growth Factor Receptor
ApplicationFlow Cytometry (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Neutralization Studies (1-2 µg/ml; see application references)
Application Data
Detection of human EGFR by immunocytochemistry. Sample: A431 cells fixed with methanol. Primary Antibody: Anti-Epidermal Growth Factor Receptor (Ab-5) Mouse mAb (Cat. No. GR15L) (1 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
BackgroundEpidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor alpha to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF-receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF-receptor is phosphorylated on tyrosine residues.
HostMouse
Immunogen speciesHuman
Immunogenpartially purified, human EGFR from A431 cells
CloneEGFR.1
IsotypeIgG2b
Specieshuman, not mouse, not rat
Positive controlA-431 cells
Negative controlHL-60 cells
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
PreservativeNone
CommentsStains A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. This antibody does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
Toxicity Standard Handling
ReferencesHunter T., 1984. Nature 311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
Application referencesNeutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511.