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MABF2774-100UG Anti-Dengue Virus 2 NS1 Antibody, clone 164

This mouse monoclonal Anti-Dengue Virus 2 NS1, clone 164, Cat. No. MABF2774, is a mouse monoclonal antibody that detects Dengue virus NS1 protein and is tested for use in ELISA.

MABF2774-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2774-100UG
DescriptionAnti-Dengue Virus 2 NS1 Antibody, clone 164
Alternate Names
  • Genome polyprotein
  • NS1 protein
  • DENV2 NS1
Background InformationGenome polyprotein (UniProt: P29990) following its synthesis is cleaved into 13 different proteins in Dengue virus 2. Dengue virus (DENV) is a mosquito-borne flavivirus and is one of the most common mosquito-transmitted viral infections. The DENV particle is about 500 nm in diameter and includes a positive-sense RNA genome with ~10,700 nucleotides and 3 structural proteins known as capsid (C), precursor membrane (prM), and envelope (E) proteins. In addition, the RNA genome of dengue virus also encodes seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) that are shown to be essential for viral replication. DENV NS1, a 48-kDa glycoprotein (aa 776-1127 of genome polyprotein), is highly conserved among all flaviviruses and is essential for viral replication. It is expressed as a monomer in infected cells and after post-translational modification in the lumen of endoplasmic reticulum, it forms homodimers associated with organelle membranes and the cell membrane. NS1 is reported to be involved in immune evasion, pathogenesis, and viral replication. Once cleaved off the polyprotein, is targeted to three destinations: the viral replication cycle, the plasma membrane and the extracellular compartment. NS1 is secreted in a hexamer form, which is composed of three dimers with a detergent-sensitive hydrophobic central cavity and it plays a role against host immune response. NS1 is shown to induce expression of sialidases, heparanase, and activates cathepsin L, which activates heparinase via enzymatic cleavage. These effects are may contribute to the endothelial hyperpermeability commonly observed in severe dengue disease. During the recovery phase, NS1 is cleared from the circulation by antibody-mediated effects. (Ref.: Chen, RU et al. (2018). J. Biomed. Sci. 25(1); 58; Bosch, I., et al. (2017). Sci. Transl. Med. 9(409); eaan1589).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationThis mouse monoclonal Anti-Dengue Virus 2 NS1, clone 164, Cat. No. MABF2774, is a mouse monoclonal antibody that detects Dengue virus NS1 protein and is tested for use in ELISA.
Key Applications
  • ELISA
Application NotesELISA Analysis: A representative lot detected Dengue Virus 2 NS1 in ELISA applications (Bosch, I., et. al. (2020). PLoS Negl Trop Dis. 14(6):e0008203).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenRecombinant Dengue Virus 2 NS1 protein.
EpitopeN-terminal half
Clone164
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 164 is a mouse monoclonal antibody that detects NS1 protein of Dengue virus 2. It targets an epitope within the N-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Virus
Species Reactivity NoteDengue Virus 2
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
UniProt Number
Molecular Weight~48 kDa calculated for NS1 protein and 379.547 kDa for the complete genome polyprotein.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA with DENV2 NS1 recombinant protein.

ELISA Analysis: 1.0 µg/mL of this antibody detected recombinant Dengue Virus 2 NS1 protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2774-100UG 04065266020663