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MABF1105 Anti-DAG Kinase Alpha Antibody, clone 10D6.1

Anti-DAG Kinase Alpha, clone 10D6.1, Cat. No. MABF1105, is a mouse monoclonal antibody that detects DAG kinase alpha. It has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-DAG Kinase Alpha Antibody, clone 10D6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: DGKA, DAGK, DAGK1 UniProt Number: P23743
MABF1105
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF1105
DescriptionAnti-DAG Kinase Alpha Antibody, clone 10D6.1
Alternate Names
  • Diacylglycerol kinase alpha
  • 80 kDa diacylglycerol kinase
  • Diglyceride kinase alpha
  • DGK-alpha
Background InformationDiacylglycerol kinase alpha (UniProt: P23743; also known as EC:2.7.1.107, DAG kinase alpha, 80 kDa diacylglycerol kinase, Diglyceride kinase alpha, DGK-alpha) is encoded by the DGKA (also known as DAGK, DAGK1) gene (Gene ID: 1606) in human. Diacylglycerol kinase is a lipid kinase that converts diacylglycerol to phosphatidic acid, and regulates the activity of many enzymes including protein kinase C, phosphatidylinositol 4-phosphate 5-kinase, and mTOR. Thus far ten mammalian subtypes of DAG kinase have been reported. All DAG kinase with the exception of beta isoform are localized in cytoplasm in several cells, but some translocate to the plasma membrane upon activation. DAG kinase acts as a modulator, which competes with protein kinase C for diacylglycerol in intracellular signaling pathways. DAG kinase also plays a role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Most DAG kinases, with the exception of theta, contain two cysteine-rich regions (C1A and C1B domains) in the regulatory domain of the N-terminal half of the molecule. In DAK kinase alpha the C1 domain closest to the catalytic domain has an extended region of fifteen amino acids that is not present in C1 domains of other DAG kinases. DAG kinase alpha can be activated by the phosphorylation of Tyr335, which occurs during T cell activation or following exposure of cells to hepatocyte growth factor.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-DAG Kinase Alpha, clone 10D6.1, Cat. No. MABF1105, is a mouse monoclonal antibody that detects DAG kinase alpha. It has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected DAG Kinase Alpha in human tonsil and human breast cancer tissue.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to the first 110 amino acids of human DAG kinase alpha.
Clone10D6.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 10D6.1 detects DAG kinase alpha in human. It targets an epitope within the first 110 amino acids from the N-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DGKA
  • DAGK
  • DAGK1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~83 kDa observed; 82.63 kDa calculaed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected DAG Kinase Alpha in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF1105 04054839224270