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CC43 Anti-Cdh1 (Ab-2) Mouse mAb (DH01)

Anti-Cdh1 (Ab-2), mouse monoclonal, clone DH01, recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells. It is validated for Western blotting and immunoprecipitation.

Anti-Cdh1 (Ab-2) Mouse mAb (DH01) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CC43
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
CC43-100UGCN Plastic ampoule 100 μg
Description
OverviewRecognizes the ~55 kDa Cdh-1 protein in Rat-1 cells.
Catalogue NumberCC43
Brand Family Calbiochem®
Application Data
Detection of human Cdh1 by immunoblotting. Sample: Human Cdh1. Primary antibody: Anti-Cdh1 (Ab-2) Mouse mAb (DH01) (Cat. No. CC43) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesKramer, E.R., et al. 2000 Mol. Biol. Cell 11, 1555.
Petersen, B.O., et al. 2000 Genes Dev. 14, 2330.
Pfleger, C.M., et al. 2001 Genes Dev. 15, 1759.
Sorensen, C.S., et al. 2001 Mol. Cell Biol. 21, 3692.
Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA 96, 11317.
Visintin, R., et al. 1997 Science 278, 460.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Negative controlNormal mouse serum
Positive controlJunkat or LS174T cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1 µg/ml)
Immunoprecipitation (2 µg/mg protein lysate)
Application CommentsDoes not cross-react with other WD repeat containing proteins, including Cdc20. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant human Cdh1 protein
ImmunogenHuman
CloneDH01
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CC43-100UGCN 04055977228472

Documentation

Anti-Cdh1 (Ab-2) Mouse mAb (DH01) SDS

Title

Safety Data Sheet (SDS) 

Anti-Cdh1 (Ab-2) Mouse mAb (DH01) Certificates of Analysis

TitleLot Number
CC43

References

Reference overview
Kramer, E.R., et al. 2000 Mol. Biol. Cell 11, 1555.
Petersen, B.O., et al. 2000 Genes Dev. 14, 2330.
Pfleger, C.M., et al. 2001 Genes Dev. 15, 1759.
Sorensen, C.S., et al. 2001 Mol. Cell Biol. 21, 3692.
Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA 96, 11317.
Visintin, R., et al. 1997 Science 278, 460.

Citations

Title
  • Kyung Uk Hong, et al. (2007) Functional importance of the anaphase-promoting complex-Cdh1-mediated degradation of TMAP/CKAP2 in regulation of spindle function and cytokinesis. Molecular and Cellular Biology 27, 3667-3681.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision21-August-2007 RFH
    ApplicationImmunoblotting (1 µg/ml)
    Immunoprecipitation (2 µg/mg protein lysate)
    Application Data
    Detection of human Cdh1 by immunoblotting. Sample: Human Cdh1. Primary antibody: Anti-Cdh1 (Ab-2) Mouse mAb (DH01) (Cat. No. CC43) (1 µg/ml). Detection: chemiluminescence.
    DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~55 kDA Cdh1 protein.
    BackgroundCdh1, a homolog of the yeast Cdc20, is a ~55 kDa protein required for the degradation of anaphase-promoting complex (APC)-dependent proteolysis. Cdh1 acts as a limiting, substrate-specific activator of APC-dependent proteolysis and it is required for the degradation of APC substrates such as Clb2 and Ase1. Cdh1 plays an important role in mitotic regulation.
    HostMouse
    Immunogen speciesHuman
    Immunogenrecombinant human Cdh1 protein
    CloneDH01
    IsotypeIgG₁
    Specieshuman
    Positive controlJunkat or LS174T cells
    Negative controlNormal mouse serum
    FormLiquid
    FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsDoes not cross-react with other WD repeat containing proteins, including Cdc20. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesKramer, E.R., et al. 2000 Mol. Biol. Cell 11, 1555.
    Petersen, B.O., et al. 2000 Genes Dev. 14, 2330.
    Pfleger, C.M., et al. 2001 Genes Dev. 15, 1759.
    Sorensen, C.S., et al. 2001 Mol. Cell Biol. 21, 3692.
    Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA 96, 11317.
    Visintin, R., et al. 1997 Science 278, 460.
    Citation
  • Kyung Uk Hong, et al. (2007) Functional importance of the anaphase-promoting complex-Cdh1-mediated degradation of TMAP/CKAP2 in regulation of spindle function and cytokinesis. Molecular and Cellular Biology 27, 3667-3681.
    		•