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MAC142 Anti-Cas9 Antibody, N-terminal Antibody, clone 6H4 Antibody, S. aureus

Anti-Cas-9, N-terminal, clone 6H4, Cat. No. MAC142, is a mouse monoclonal antibody that detects Cas-9 in Staphylococcus aureus and its identity has been confimed by Isotyping Test and Western Blotting.

MAC142
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMAC142
DescriptionAnti-Cas9 Antibody, N-terminal Antibody, clone 6H4 Antibody, S. aureus
Alternate Names
  • SaCas9
  • CRISPR-associated endonuclease Cas-9
Background InformationCRISPR-associated endonuclease Cas9 (UniProt: J7RUA5; also known as SaCas9) is encoded by the Cas9 gene (Gene ID: in Staphylococcus aureus. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer.Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self-versus non-self, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity. Cas9 endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3'-5' exonucleolytically.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Cas-9, N-terminal, clone 6H4, Cat. No. MAC142, is a mouse monoclonal antibody that detects Cas-9 in Staphylococcus aureus and its identity has been confimed by Isotyping Test and Western Blotting.
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Immunofluorescence
Application NotesWestern Blotting Analysis: 0.57 µg/mL from a representative lot detected CRISPR-associated endonuclease Cas9/Csn1 in whole cell lysates of HEK293 (WT), HEK293/Cas9 from S. aureus, or HeLa/Cas9 from S. pyogenes. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected CRISPR-associated endonuclease Cas9/Csn1 in transiently transfected U20S cells expressing SaCas9-T2A-GFP. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Immunoprecipitation Analysis: 1-2 µg from a representative lot detected CRISPR-associated endonuclease Cas9/Csn1 in SaCas9. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Biological Information
ImmunogenHis-tagged recombiant Cas-9 from Staphylococcus aureus.
EpitopeN-terminus
Clone6H4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 6H4 detects Cas-9 in Staphylococcus aureus.
IsotypeIgG2bκ
Species Reactivity
  • Bacteria
  • All
Species Reactivity NoteStaphylococcus aureus.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Cas9
Purification MethodProtein G purified
UniProt Number
Molecular Weight~124 kDa observed; 123.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIdentity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAC142 04054839233135

Documentation

Anti-Cas9 Antibody, N-terminal Antibody, clone 6H4 Antibody, S. aureus SDS

Title

Safety Data Sheet (SDS) 

Anti-Cas9 Antibody, N-terminal Antibody, clone 6H4 Antibody, S. aureus Certificates of Analysis

TitleLot Number
Anti-Cas9, N-terminal, clone 6H4, S. aureus Monoclonal Antibody 3078409
Anti-Cas9, N-terminal, clone 6H4, S. aureus - 3455241 3455241
Anti-Cas9, N-terminal, clone 6H4, S. aureus Monoclonal Antibody Q2979665
Anti-Cas9, N-terminal, clone 6H4, S. aureus Monoclonal Antibody Q2871721

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