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MABS1291 Anti-Cas9 Antibody, C-term. clone 10C11-A12

Anti-Cas9 Antibody, C-term. clone 10C11-A12 is an antibody against Cas9 for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-Cas9 Antibody, C-term. clone 10C11-A12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: cas9, csn1 UniProt Number: Q99ZW2
MABS1291
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
BactICC, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1291
DescriptionAnti-Cas9 Antibody, C-term. clone 10C11-A12
Alternate Names
  • CRISPR-associated endonuclease Cas9/Csn1
  • SpyCas9
Background InformationCRISPR-associated endonuclease Cas9/Csn1 (UniProt Q99ZW2; also known as SpyCas9) is encoded by the Streptococcus pyogenes serotype M1 gene cas9 (also known as csn1) gene (Gene ID 901176). Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA loci containing short repetitions of base sequences complementary to antecedent mobile elements and target invading nucleic acids. Each repetition is followed by short segments of spacer DNA from previous exposures to a virus. Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1's CRISPR immune system that confers resistance to foreign genetic elements such as plasmids and phages. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems, correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc), and Cas9. Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3'-5' exonucleolytically.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Cas9 Antibody, C-term. clone 10C11-A12 is an antibody against Cas9 for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunocytochemistry Analysis: 33.5 µg/mL from a representative lot detected doxycycline-induced expression of exogenously transfected S. pyogenes Cas9 construct under the control of the PTight (Tet-ON) promoter by fluorescent immunocytochemistry staining of methanol-fixed, acetone-permeabilized HeLa transfectants (Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria).
Immunoprecipitation Analysis: 13.4 µg from a representative lot immunoprecipitated exogenously expressed Cas9 in 250 µg of lysate from transfected, but not untransfected, HeLa cells (Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria).
Western Blotting Analysis: 1.675 µg/mL from a representative lot detected exogenously expressed Cas9 in 20 µg of lysate from transfected, but not untransfected, HeLa cells (Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria).
Western Blotting Analysis: Clone 10C11-A12 hybridoma culture supernatant detected doxycycline treatment-induced expression of a FLAG-tagged Cas9 construct under the control of the PTight promoter in a HeLa-derived Tet-ON cell line (Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria).
Biological Information
ImmunogenHis-tagged recombinant S.pyogenes Cas9 C-terminal fragment.
EpitopeC-terminal region.
Clone10C11-A12
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 10C11-A12 recognizes an epitope located in the C-terminal region of Cas9.
IsotypeIgG1κ
Species Reactivity
  • Bacteria
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • cas9
  • csn1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~158 kDa observed. 158.4 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Cas9-transfected HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected the exogenously expressed Cas9 in 10 µg lysate from transfected HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1291 04055977335224

Documentation

Anti-Cas9 Antibody, C-term. clone 10C11-A12 SDS

Title

Safety Data Sheet (SDS) 

Anti-Cas9 Antibody, C-term. clone 10C11-A12 Certificates of Analysis

TitleLot Number
Anti-Cas9, C-term. Clone 10C11-A12 - 3438027 3438027
Anti-Cas9, C-term. Clone 10C11-A12 - 3541410 3541410
Anti-Cas9, C-term. Clone 10C11-A12 - 3905431 3905431
Anti-Cas9, C-term. Clone 10C11-A12 -2802543 2802543
Anti-Cas9, C-term. Clone 10C11-A12 -Q2671475 Q2671475
Anti-Cas9, C-term. Clone 10C11-A12 Monoclonal Antibody 2930963
Anti-Cas9, C-term. Clone 10C11-A12 Monoclonal Antibody 3009983
Anti-Cas9, C-term. Clone 10C11-A12 Monoclonal Antibody 2907824
Anti-Cas9, C-term. Clone 10C11-A12 Monoclonal Antibody 3099062
Anti-Cas9, C-term. Clone 10C11-A12 Monoclonal Antibody - 4042766 4042766