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178612 Anti-Aquaporin 2 (254-271) Rabbit pAb

Anti-Aquaporin 2 (254-271), rabbit polyclonal, recognizes the ~40 and ~29 kDa forms of aquaporin-2 in rat kidney membrane. Supplied with a control peptide. It is validated for WB, IHC, and IP.

Anti-Aquaporin 2 (254-271) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-AQP2, Anti-AQP-CD, Anti-WCH-CD

178612
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
178612-1STCN Plastic ampoule 1 st
Description
OverviewRecognizes the ~40 kDa and ~29 kDa forms of aquaporin-2 in rat kidney membrane. Supplied with a control peptide (AQP2254-271 immunogen peptide).
Catalogue Number178612
Brand Family Calbiochem®
SynonymsAnti-AQP2, Anti-AQP-CD, Anti-WCH-CD
Application Data
Detection of rat aquaporin 2 by immunoblotting. Samples: Rat kidney membranes (lane 1) and preincubated with a control peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:200). Detection: chemiluminescence.

Detection of rat aquaporin 2 by immunohistochemistry. Sample: Rat kidney showing the inner medulla. Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:100). Detection: fluorescence.
References
ReferencesFushimi, K., et al. 1997. J. Biol. Chem. 272, 14800.
Fushimi, K., et al. 1993. Nature 361, 549.
Ma, T., et al. 1993. Biochem. Biophys. Res. Commun. 197, 654.
Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 11663.
Product Information
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, 5% sucrose, pH 7.4 and 40 µg control peptide lyophilized from PBS.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoprecipitation Fushimi, K., et al. 1997. J. Biol. Chem. 272, 14800. Immunohistochemistry Fushimi, K., et al. 1993. Nature 361, 549. Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 11663.
Key Applications Immunoblotting (Western Blotting)
Immunohistochemistry
Immunoprecipitation
Application NotesImmunoblotting (1:200-1:600)
Immunohistochemistry (see application reference)
Immunoprecipitation (see application reference)
Application CommentsImmunogen peptide is identical in rat and mouse protein, and differs by one amino acid in the human sequence. The epitope is unique for AQP2 and is not present in other known related proteins. It is identical in mouse and rat, and only slightly different (17/18 identical) in human. To prepare a negative control, preincubate 1 µg peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide [(KY)RQSVELHSPQSLPRGSKA] corresponding to amino acids 254-271 of rat and mouse AQP2, conjugated to KLH
ImmunogenMouse/Rat
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsPlease see antibody label for lot specific reconstitution instructions. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Following reconstitution, aliquot and freeze (-20°C). Reconstitute the peptide in 100 µl deionized water, aliquot, and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
178612-1STCN 04055977204902

Documentation

Anti-Aquaporin 2 (254-271) Rabbit pAb Certificates of Analysis

TitleLot Number
178612

References

Reference overview
Fushimi, K., et al. 1997. J. Biol. Chem. 272, 14800.
Fushimi, K., et al. 1993. Nature 361, 549.
Ma, T., et al. 1993. Biochem. Biophys. Res. Commun. 197, 654.
Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 11663.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-February-2008 JSW
SynonymsAnti-AQP2, Anti-AQP-CD, Anti-WCH-CD
ApplicationImmunoblotting (1:200-1:600)
Immunohistochemistry (see application reference)
Immunoprecipitation (see application reference)
Application Data
Detection of rat aquaporin 2 by immunoblotting. Samples: Rat kidney membranes (lane 1) and preincubated with a control peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:200). Detection: chemiluminescence.

Detection of rat aquaporin 2 by immunohistochemistry. Sample: Rat kidney showing the inner medulla. Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:100). Detection: fluorescence.
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~40 kDa and ~29 kDa aquaporin-2 proteins. Supplied with a control peptide (AQP2254-271 immunogen peptide).
BackgroundAQP2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. Defects in AQP2 are a cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI).
HostRabbit
Immunogen speciesMouse/Rat
Immunogena synthetic peptide [(KY)RQSVELHSPQSLPRGSKA] corresponding to amino acids 254-271 of rat and mouse AQP2, conjugated to KLH
IsotypeIgG
Specieshuman, mouse, rat
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, 5% sucrose, pH 7.4 and 40 µg control peptide lyophilized from PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsImmunogen peptide is identical in rat and mouse protein, and differs by one amino acid in the human sequence. The epitope is unique for AQP2 and is not present in other known related proteins. It is identical in mouse and rat, and only slightly different (17/18 identical) in human. To prepare a negative control, preincubate 1 µg peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsPlease see antibody label for lot specific reconstitution instructions. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Following reconstitution, aliquot and freeze (-20°C). Reconstitute the peptide in 100 µl deionized water, aliquot, and freeze (-20°C).
Toxicity Irritant
ReferencesFushimi, K., et al. 1997. J. Biol. Chem. 272, 14800.
Fushimi, K., et al. 1993. Nature 361, 549.
Ma, T., et al. 1993. Biochem. Biophys. Res. Commun. 197, 654.
Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 11663.
Application referencesImmunoprecipitation Fushimi, K., et al. 1997. J. Biol. Chem. 272, 14800. Immunohistochemistry Fushimi, K., et al. 1993. Nature 361, 549. Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 11663.