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ABS2247-100UG Anti-Acetyl-Miro1 (Lys 105)

Anti-Acetyl-Miro1 (Lys 105), Cat. No. ABS2247, is a rabbit polyclonal antibody that detects mitochondrial Rho GTPase (Miro) acetylated on Lysine 105 and is tested for use in ELISA, Immunofluorescence, and Western Blotting.

ABS2247-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABS2247-100UG
DescriptionAnti-Acetyl-Miro1 (Lys 105)
Alternate Names
  • Mitochondrial Rho GTPase
Background InformationMitochondrial Rho GTPase (UniProt: A1L1L6; also known as Miro1) is encoded by the Rhot1 (also known as Rnf135, rCG34120) gene (Gene ID: 303351) in rat. Miro1 is a single-pass type IV membrane protein with a transmembrane domain (aa 605-627). It functions as a mitochondrial GTPase and is involved in mitochondrial trafficking and is considered to be a potential adaptor for microtubule‐based peroxisome motility in mammalian cells. It plays a key role in mitochondrial motility on microtubules and in homeostasis. In mammals, Miro1 and Miro2 share about 60% sequence homology. Three acetylation sites for Miro1 have been identified in rat that are located on lysines 105, 525, and 629. Acetylation of Miro1 on lysine 105 (K105) is shown to increase mitochondrial transport and axon growth. Exposure of neurons to myelin-associated glycoprotein (MAG) and chondroitin sulfate proteoglycans reduces K105 acetylation of Miro1, which reduces axonal mitochondrial transport. Ac-Miro1 undergoes deacetylation with histone deacetylase 6 (HDAC6) and inhibition of HDAC6 increases Miro1 acetylation in axons. Loss of Miro1 in neurons causes depletion of mitochondria from corticospinal tract axons and leads to progressive neurological deficits similar to upper motor neuron disease. (Ref.: Kalinkski, AL., et al. (2019). J. Cell Biol. 218(6); 1871-1890; Castro, IG., et al. (2018). Traffic. 19(3); 229-242; Nguyen, TT., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(35); E3631-E3640).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Acetyl-Miro1 (Lys 105), Cat. No. ABS2247, is a rabbit polyclonal antibody that detects mitochondrial Rho GTPase (Miro) acetylated on Lysine 105 and is tested for use in ELISA, Immunofluorescence, and Western Blotting.
Key Applications
  • Western Blotting
  • Enzyme Immunoassay (ELISA)
  • Immunofluorescence
Application NotesTested Applications
Evaluated by Western Blotting in Rat cortical neurons treated with Tubastatin A.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected lysine 105 acetylated Mitochondrial Rho GTPase (Miro) in lysate from rat cortical neurons treated with Tubastatin A (10 µM; 4 h)
Immunofluorescence Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in Immunofluorescence applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).
Western Blotting Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in Western Blotting applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).
ELISA Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in ELISA applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenLinear peptide corresponding to 10 amino acids surrounding acetylated lysine 105 from the N-terminal half of rat Mitochondrial Rho GTPase (Miro1).
EpitopeN-terminal half
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects mitochondrial Rho GTPase acetylated on lysine 105. It targets an epitope within 10 amino acids surrounding acetylated lysine 105 from the N-terminal half.
IsotypeIgG
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Human, Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Rhot1
  • Rnf135
  • rCG34120
Modifications
  • Acetylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~54 kDa observed; 72.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat cortical neurons treated with Tubastatin A.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected lysine 105 acetylated Mitochondrial Rho GTPase (Miro) in lysate from rat cortical neurons treated with Tubastatin A (10 µM; 4 h)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS2247-100UG 04065266298239