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MABF2078-25UG Anti-APN/CD13 Antibody, clone DF-5

This mouse monoclonal Anti-APN/CD13, clone DF-5, Cat. No. MABF2078, detects Aminopeptidase N and has been tested for use in Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Anti-APN/CD13 Antibody, clone DF-5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: ANPEP, APN, CD13, PEPN UniProt Number: P15144
MABF2078-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICC, IF, IHC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2078-25UG
DescriptionAnti-APN/CD13 Antibody, clone DF-5
Alternate Names
  • EC:3.4.11.2
  • AP-N
  • hAPN
  • Alanyl aminopeptidase
  • Aminopeptidase M
  • AP-M
  • Microsomal aminopeptidase
  • Myeloid plasma membrane glycoprotein CD13
  • gp150
  • CD13
Background InformationAminopeptidase N (UniProt: P15144; also known as EC:3.4.11.2, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD13) is encoded by the ANPEP (also known as APN, CD13, PEPN) gene (Gene ID: 290) in human. Aminopeptidase N is a single-pass type II membrane protein that is also found as a soluble form in cells. It is widely expressed as a homodimer of 280 kDa on the cell surface in many tissues, including intestinal epithelia and the nervous system. It serves as a broad specificity aminopeptidase that plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. It preferentially cleaves N-terminus neutral amino acids, most notably alanine residue. Aminopeptidase N is involved in many physiological processes, including antigen presentation regulation, differentiation, proliferation, apoptosis, cancer metastasis, and angiogenesis. It is also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Human Aminopeptidase N consists of a short N-terminal cytoplasmic end (aa 2-8), a transmembrane domain (aa 9-32), and a large extracellular portion (aa 33-967), which is composed of a Ser/Thr-rich region (aa 33-68) and the metalloprotease domain (aa 69-967).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-APN/CD13, clone DF-5, Cat. No. MABF2078, detects Aminopeptidase N and has been tested for use in Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected APN/CD13 in human prostate tissue.

Flow Cytometry Analysis: A representative lot detected APN/CD13 in Flow Cytometry applications (Sorrell, J.M., et. al. (2003). Exp Dematol. 12(3):315-23).

Immunofluorescence Analysis: A representative lot detected APN/CD13 in Immunofluorescence applications (Sorrell, J.M., et. al. (2003). Exp Dematol. 12(3):315-23; Sorrell, J.M., et. al. (2007). Cell Tissue Res. 327(3):499-510).

Western Blotting Analysis: A representative lot detected APN/CD13 in Western Blotting applications (Sorrell, J.M., et. al. (2003). Exp Dematol. 12(3):315-23).

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected APN/CD13 in PC3 cell line.
Biological Information
ImmunogenCrude cell membrane fraction from adult human papillary and reticular dermal fibroblasts.
CloneDF-5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone DF-5 specifically detects aminopeptidase N in human cells.
IsotypeIgG3κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ANPEP
  • APN
  • CD13
  • PEPN
Purification MethodProtein G purified
UniProt Number
Molecular Weight109.54 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human kidney tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected APN/CD13 in human kidney tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2078-25UG 04054839431906