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CBA059 Annexin V-FITC Apoptosis Detection Kit II

This annexin FITC Apoptosis detection kit is a highly sensitive and specific fluorometric assay kit for measuring Annexin V in apoptotic cells.

Annexin V-FITC Apoptosis Detection Kit II MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CBA059
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Overview

Key Spec Table

Detection Methods
Fluorescence

Products

Catalogue NumberPackaging Qty/Pack
CBA059-1KITCN Glass bottle 1 kit
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Description
OverviewA convenient kit for the identification of changes in the plasma membrane that occur during apoptosis. In apoptotic cells, phosphoatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, allowing for the detection of PS on the cell surface. In the presence of Ca 2+, Annexin V exhibits a high affinity for PS and binds cells with exposed PS. Annexin V, when conjugated to a fluorophore, can be used to monitor apoptopic cells using flow cytometry or immunofluorescence microscopy.
Catalogue NumberCBA059
Brand Family Calbiochem®
Materials Required but Not Delivered• 1X PBS
• Flow cytometer or fluorescence microscope
References
ReferencesDarzynkiewicz, Z., et al. 1997. Cytometry 27, 1.
Frey, T. 1997. Cytometry 28, 253;
Boersma, A.W.M., et al. 1996. Cytometry 24, 123.
White, E. 1996. Genes and Devel. 10, 1.
Martin, S.J., et al. 1995. J. Exp. Med. 182, 1545.
Koopman, G., et al. 1994. Blood 84, 1415.
Wyllie, A.H.,1993. Br. J. Cancer 67, 205.
Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
Fadok, V.A., et al. 1992. J. Immunology 148, 2207.
Schmid, I., et al. 1992. Cytometry 13, 204.
Kerr, J.F.R., et al. 1972. Cancer 26, 239.
Product Information
Detection methodFluorescence
Form20 Tests
FormatFlow cytometry or fluorescence microscopy
Kit containsAnnexin V-FITC, Binding Buffer, Propidium Iodide and a user protocol.
Quality LevelMQ100
Applications
Key Applications Flow Cytometry
Immunofluorescence
Biological Information
Assay time30 min
Sample TypeIntact cells
Product Usage Statements
Intended useThe Calbiochem® Annexin V-FITC Apoptosis Detection Kit II is a non-isotopic system that can be used to detect phosphatidylserine on the outer leaflet of the cell membrane of apoptotic cells using flow cytometry or fluorescence microscopy.
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage +2°C to +8°C
Storage ConditionsUpon arrival store the entire contents of the kit at 4°C.
Do not freeze Ok to freeze
Supplemental Information
Kit containsAnnexin V-FITC, Binding Buffer, Propidium Iodide and a user protocol.
Global Trade Item Number
Catalogue Number GTIN
CBA059-1KITCN 07790788053666