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287815 3,4-Dichloroisocoumarin - CAS 51050-59-0 - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: serine proteases
287815
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
51050-59-0C₉H₄Cl₂O₂

Products

Catalogue NumberPackaging Qty/Pack
287815-10MGCN Plastic ampoule 10 mg
Description
OverviewA potent, irreversible inhibitor of serine proteases. Reacts with serine proteases to release an acylchloride moiety that can acylate another active site residue. Inhibits granzyme B and blocks apoptotic internucleosomal DNA cleavage in thymocytes without the involvement of endonucleases. Does not affect thiol proteases and metalloproteases. Does not exhibit any activity towards β-lactamases.
Effective concentration: 5-100 µM; t½ = 20 min at pH 7.5.
Catalogue Number287815
Brand Family Calbiochem®
References
ReferencesCain, K., et al. 1994. Biochem. Cell Biol. 72, 631.
Weaver, V.M., et al. 1993. Biochem. Cell Biol. 71, 488.
Odake, S., et al. 1991. Biochemistry 30, 2217.
Harper, J.W., et al. 1985. Biochemistry 24, 1831.
Product Information
CAS number51050-59-0
ATP CompetitiveN
FormWhite lyophilized solid
Hill FormulaC₉H₄Cl₂O₂
Chemical formulaC₉H₄Cl₂O₂
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary Targetserine proteases
Primary Target IC<sub>50</sub>5-100 µM
Purity≥99% by elemental analysis
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
287815-10MGCN 04055977197860

Documentation

3,4-Dichloroisocoumarin - CAS 51050-59-0 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

3,4-Dichloroisocoumarin - CAS 51050-59-0 - Calbiochem Certificates of Analysis

TitleLot Number
287815

References

Reference overview
Cain, K., et al. 1994. Biochem. Cell Biol. 72, 631.
Weaver, V.M., et al. 1993. Biochem. Cell Biol. 71, 488.
Odake, S., et al. 1991. Biochemistry 30, 2217.
Harper, J.W., et al. 1985. Biochemistry 24, 1831.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
  • Dong Zhang, et al. (2001) Granzymes A and B Directly Cleave Lamins and Disrupt the Nuclear Lamina During Granule-Mediated Cytolysis. Proceedings of the National Academy of Science USA 98, 5746-5751.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision17-June-2008 RFH
    DescriptionA potent irreversible inhibitor of serine proteases. Blocks apoptotic internucleosomal DNA cleavage in thymocytes without the involvement of endonucleases. Reacts with serine proteases to release an acylchloride moiety that can acylate another active site residue. Does not affect thiol proteases and metalloproteases. Also has no activity towards β-lactamases. Effective at concentrations ranging from 5-100 µM.
    FormWhite lyophilized solid
    CAS number51050-59-0
    Chemical formulaC₉H₄Cl₂O₂
    Structure formulaStructure formula
    Purity≥99% by elemental analysis
    SolubilityDMF (10 mM) or DMSO (10 mM)
    Storage Protect from moisture
    +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
    Toxicity Standard Handling
    ReferencesCain, K., et al. 1994. Biochem. Cell Biol. 72, 631.
    Weaver, V.M., et al. 1993. Biochem. Cell Biol. 71, 488.
    Odake, S., et al. 1991. Biochemistry 30, 2217.
    Harper, J.W., et al. 1985. Biochemistry 24, 1831.
    Citation
  • Dong Zhang, et al. (2001) Granzymes A and B Directly Cleave Lamins and Disrupt the Nuclear Lamina During Granule-Mediated Cytolysis. Proceedings of the National Academy of Science USA 98, 5746-5751.
    		•