- LiChrolut® EN (40 - 120 µm) for solid phase extraction in environmental analysis
- LiChrolut® EN (40 - 120 µm) 100 mg (bottom) LiChrolut® RP-18 (40 - 63 µm) 200 mg (top) 6 ml standard PP-tubes 30 pieces
- LiChrolut® EN (40 - 120 µm) 200 mg 3 ml standard glass tubes 30 pieces
- LiChrolut® EN (40 - 120 µm) 200 mg 6 ml standard PP-tubes 30 pieces
- LiChrolut® EN (40 - 120 µm) 500 mg 6 ml standard PP-tubes 30 pieces
- LiChrolut® EN 200 mg 3 ml standard PP-tubes 30 pieces
- Adapter (PTFE) Luer inlet for solvent reservoir, for LiChrolut® columns of various sizes
- Disposable PTFE liner for LiChrolut® vacuum manifold
- Enrichment capillaries for large-volume samples
- Frits (PTFE) for 3 ml glass columns, porosity 10 µm LiChrolut®
- Glass columns (empty) 3 ml compatible with SPE robot systems LiChrolut®
- LiChrolut® Drying unit for sample preparation
- Vacuum manifold for sample preparation LiChrolut®
- Exit cannulas for EXtrelut® NT 1 and EXtrelut® NT 3
LiChrolut® EN |
Application Examples
LiChrolut® EN is a mixed polarity copolymer, which provides both polar and non-polar interaction sites, hence it is perfectly suited for solid-phase extraction of mixed polar analytes. Thanks to its extremely high surface area of 1,200-1,400 m2/g, the sorbent is ideal for trace analysis of polar compounds. Furthermore, LiChrolut® EN delivers outstanding performance for applications at extreme pH conditions between pH 1 and 13.
Typical Applications
| Analytes | Environmental pollutants: Fungicides, herbicides, phenols, pesticides, parabens, hydrocarbons Pharmaceuticals: Antibiotics, barbiturates, benzodiazepines, caffeine, drugs and their metabolites Food & beverage: Dyes, essential oils, organic acids, fat/water soluble vitamins, steroids, phthalate esters, surfactants, theophylline |
|---|---|
| Matrix | Mostly polar: aqueous buffer, serum, plasma, urine, beverages, environmental samples (waste/drinking water, soil) |
| Eluents | Organic solvents: alcohols, acetonitrile, n-hexane, methylene chloride, ethyl acetate |
Sample preparation of drinking water specimens
| Anilines pH 9 with NaOH | Explosives pH 5.5-6.0 | Solid-phase extraction LiChrolut® EN 200 mg, 3 mL | Pesticides pH 5.5-6.0 | Phenols pH with 25 % HCl |
|---|---|---|---|---|
| 3 mL ethyl acetate 3 mL methanol 3 mL water |
3 mL methanol 3 mL water |
Conditioning | 3 mL methanol 3 mL water |
3 mL ethyl acetate 9 mL water, pH 2 |
| 1000 mL sample within 2 h | 1000 mL sample within 2 h | Sample application | 1000 mL sample within 2 h | 1000 mL sample within 2 h |
| 1 mL water | Not required | Washing | 1 mL water | 1 mL water, pH 2 |
| 1 min with nitrogen | Not required | Drying | 10 min with nitrogen | 5 min with nitrogen |
| 2 x 1.5 mL methanol / acetonitrile / acetone (50/50/1) | 2 x 1.5 mL acetontrile / methanol | Elution | 2 x 3 mL methanol / ethyl acetate | 3 x 0.3 mL ethyl acetate |
Pesticide recovery rates of samples of tap water (N = 10) containing the 33-multicomponent standard (c = 200 ng/l per pesticide)
| Pesticide | Pesticide recovery rate ± rsd (%) |
| 1. Desisopropylatrazine 2. Metamitron 3. Chloridazon 4. Desethylatrazine 5. Crimidine 6. Carbetamide 7. Bromacil 8. Simazine 9. Cyanazine 10. Desethylterbutylazine 11. Karbutilate 12. Methabenzthiazuron 13. Chlortoluron 14. Atrazine 15. Monolinuron 16. Isoproturon 17. Diuron 18. Metobromuron 19. Metazachlor 20. Methoprotryne 21. Dimefuron 22. Sebutylazine 23. Propazine 24. Terbutylazine 25. Linuron 26. Chloroxuron 27. Prometryne 28. Chlorpropham 29. Terbutryne 30. Metolachlor 31. Pencycuron 32. Bifenox 33. Pendimethalin |
100 ± 2.7 98 ± 1.4 96 ± 1.8 101 ± 2.6 86 ± 3.2 87 ± 3.8 103 ± 3.4 99 ± 1.7 100 ± 1.9 95 ± 2.2 82 ± 4.7 94 ± 2.4 100 ± 2.5 100 ± 3.8 98 ± 1.8 101 ± 3.8 102 ± 5.0 99 ± 3.2 108 ± 5.6 99 ± 3.8 100 ± 1.7 99 ± 1.7 102 ± 1.9 98 ± 1.5 97 ± 1.9 101 ± 1.1 95 ± 2.3 101 ± 2.8 96 ± 1.6 102 ± 1.5 91 ± 2.5 102 ± 4.1 98 ± 5.0 |
