Chromolith® RP-18 Endcapped HPLC Columns

Ultra-High Performance on Any Instrument

Chromolith® 2 mm i.d. (internal diameter) columns for liquid chromatography offer an unparalleled combination of ultra-high performance and exceptionally low operating pressure. This leads to ultra-fast results, not only in the new UHPLC and UPLC instruments, but equally well in all standard HPLC systems with low dead volume.

The columns owe their rapid separation speed to their unique bimodal pore structure. The macropores have a diameter of 1.5 μm, resulting in a column efficiency that exceeds 100,000 plates/meter. Whereas the 13 nm (130 Å) mesopores are responsible for the large, uniform surface area on which adsorption takes place. Furthermore, Chromolith® RP-18 endcapped 2 mm i.d. columns have octadecylsilane surface modification, and are fully endcapped.

Features and Benefits

• Flexibility: Very fast, high-performance results with all low dead-volume LC instruments (UHPLC, UPLC, standard HPLC)
• Increased sensitivity and solvent efficiency: 81% solvents saved and 5.7 times higher sensitivity (compared to 4.6 mm i.d. columns)
• Flow rates from 0.2 - 1 mL/min: Compatible with LC-MS systems, and ESI or APCI interfaces 
• Long column lifetime and resistance to blocking: Monolithic silica structure and absence of frits ensure method robustness and cost savings

Technical Data

Chromolith® RP-18 Endcapped 2 mm i.d. Columns

Application Examples

Chromolith® Performance RP-18 endcapped 100-4.6 mm

Column	Chromolith® Performance RP-18 endcapped 100-4.6 mm
Mobile Phase	A: 100% Acetonitrile B: 100% Water + 0.1% TFA (v/v) C: 100% Methanol
Isocratic	Initial composition: A/B/C 30/60/10 (v/v/v)
Flow rate	2 mL/min
Pressure	45 bar (4.5 MPa, 65.3 psi)
Detection	Dionex Ultimate 3000 VWD-3400, 2.5 Hz, Response time 0.1 s, UV = 210 nm
Vol. detector cell	11 μL
Temp.	Ambient
Injection volume	1 μL
Sample	Bimatoprost Bimatoprost free acid

Chromolith® Performance RP-18 endcapped 100-2 mm

Column	Chromolith® Performance RP-18 endcapped 100-2 mm
Mobile Phase	A: 100% Acetonitrile B: 100% Water + 0.05% TFA (v/v) C: 100% Methanol
Isocratic	Initial composition: A/B/C 30/60/10 (v/v/v)
Flow rate	380 μL/min
Pressure	48 bar (4.8 MPa, 70 psi)
Detection	Dionex Ultimate 3000 VWD-3400, 2.5 Hz, Response time 0.1 s, UV = 210 nm
Vol. detector cell	1.4 μL
Temp.	Ambient
Injection volume	1 μL
Sample	Bimatoprost Bimatoprost free acid

Separation of alkylphenones

Column	Chromolith® Performance RP-18 endcapped 100-2 mm
Mobile Phase	A: Acetonitrile B: Water
Gradient	Time	%ACN	%Water
	0	15	85
	3.5	90	10
	5	90	10
	5.1	15	85
	6	15	85
Flow rate	0.38 mL/min
Pressure	37-79 bar
Detection	254 nm
Temperature	Ambient
Injection volume	0.5 μL
Sample	1. Thiurea 2. Acetanilide 3. Acetophenone 4. Propiophenone 5. Benzophenone 6. Butyrophenone 7. Valerophenone 8. Hexanophenone 9. Heptanophenone in ACN/Water 60/40

Ultra-fast separation of antihistamines

Column	Chromolith® FastGradient RP-18 endcapped 50-2 mm
Mobile Phase	A: 0.1% TFA in water B: 0.1% TFA in ACN
Gradient	5% to 90% B in 3.4 min
Flow rate	1.0 mL / min
Pressure	50 -120 bar
Detection	UV 230 nm
Temp.	Ambient
Injection volume	0.2 μL
Sample	1. Phenylephrine 2. Tripelenamine 3. Pyrilamine 4. Chloropheniramine 5. Brompheniramine 6. Chloropyramine 7. Diphenhydramine 8. Promethazine 9. Loratadine 10. Meclizine

Carotenoids of salmon: Fast separation with Chromolith® Flash RP-18e 25-2 mm

Column	Chromolith® Flash RP-18 endcapped 25-2 mm
Mobile Phase	A: Acetonitrile B: Water + 0.1% Formic acid
Gradient	Time	%ACN	%Water
	0	90	10
	3	50	50
Flow rate	1.14 mL/min
Pressure	22-53 bar
Detection	436 nm 11 μL flow cell
Temperature	Ambient
Injection volume	5 μL
Sample	1) Astaxanthin (cis + trans) 2) Canthaxanthin cis + trans) dissolved in acetonitrile/water + 0.1% formic acid 2/1

Bioflavonid separation: UHPLC with Chromolith® Performance RP-18e 100-2 mm

Column	Chromolith® Performance RP-18 endcapped 100-2 mm
Mobile Phase	A: 0.1% TFA in H2O B: MeOH
Gradient	t [min]	A [%]	B [%]	flow [mL/min]
	0.0	85	15	0.50
	2.5	50	50	0.50
	5.0	50	50	0.50
	5.1	85	15	0.50
	8.5	85	15	0.50
Detection	220 nm UV
Temperature	Ambient
Injection volume	0.5 μL
Sample	1. Isoquerecetin 2. Troxerutin 3. Naringin 4. Morin 5. Querecetin 6. Trihydroxyethylluteolin

Proteomics

Column	Chromolith® Performance RP-18 endcapped 100-2 mm
Mobile Phase	A: 95% H2O/5% ACN/0.1% TFA (v/v/v) B: 5% H2O/95% ACN/0.085% TFA (v/v/v)
Gradient	from 5% B to 50% B in 20 min
Flow rate	0.3 mL/min
Detection	UV 214 nm
Sample	1 μL BSA digest (1mg/mL)