On-Demand Webinar

The Importance of Media Selection and Scale-down Models for High-titer Expression in CHO Cells

Duration: 1 Hour
Speaker: Sebastien Ribault, Director, Bioproduction & Development, Merck

Abstract: The quest for a viable industrial biotechnology process generally starts with the generation of a cell line expressing the protein of interest. In many cases, the search for the best-producing clone is often like looking for a needle in a haystack. While clonal selection is emphasized, the metabolic aspect is often neglected and optimization of the media and associated feeds used for the upstream process is less of a focus.

Most companies spend several months, if not years, developing their own generic medium and feed platforms, or have opted to use commercial ones. Medium and feed platform assessment is being performed earlier in the product development process and clone screening is conducted increasingly in fed-batch conditions. Media selection is a crucial step as is the ability to use the first data generated at very small scale (such as spin tubes) to define large-scale behavior. Therefore, it is important to have the right screening tools with lower working volumes available.

With so many variants of the CHO cell line, generic media and associated supplements, it is difficult to select one media and associate it with a CHO-derived cell line. We have found that screening 3 to 6 clones against 5 to 10 media is an effective way to find the best clone/media/feed combination.

This webinar will assess our screening scale-down model through a “generic” CHO media assessment. We will define the conditions of a standardized platform, allowing us to scale up from spin tubes to 3L and then to 200 L. A comparison of the various scales used in this template will be presented.

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