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69076 pET-32 Ek/LIC Vector Kit - Novagen

Novagen's pET-32 Ek/LIC vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx-Tag thioredoxin protein and His-Tag and S-Tag thioredoxin protein

pET-32 Ek/LIC Vector Kit - Novagen: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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69076
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      Description
      OverviewThe pET-32 Ek/LIC vector is an Ek/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag™ thioredoxin protein, and His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Ek protease cleavage site (AspAspAspAspLys↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification.




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69076
      Brand Family Novagen®
      Application Data
      References
      Product Information
      Components
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      69076-3 04055977259537

      Documentation

      pET-32 Ek/LIC Vector Kit - Novagen Certificados de análisis

      CargoNúmero de lote
      69076

      Folleto

      Cargo
      Radiance! Multisystem Protein Expression

      Citas

      Título
    • Xuezhi Bi, Gurdev S. Khush and John Bennett. (2005) The rice nucellin gene orthologue, OsAsp1 which encodes an active aspartic protease without plant-specific insert, is strongly expressed in early embryo. Plant and Cell Physiology 46, 87-98.
    • Hamid Mehenni, et al. (2005) LKB1 interacts with and phosphorylates PTEN: a functional link between two proteins involved in cancer predisposing syndromes. Human Molecular Genetics 14, 2209-2219.
    • John V. McDowell, et al. (2004) Demonstration of the involvement of outer surface protein E coiled coil structural domains and higher order structural elements in the binding of infection-induced antibody and the complement-regulatory protein, factor H. Journal of Immunology 173, 7471-7480.
      		•

      Protocolos de usuario

      Cargo
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual
      TB163 Ek/LIC Cloning Kits
      TB184 Xa/LIC Cloning Kits

      Mapa vectorial

      Cargo
      TB161VM pET-32 Ek/LIC Vector Map

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      Productos relacionados por: Application Facete

      PCR and Cloning

      Categorías

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors