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596105 THIOLYTE® Monobromobimane Reagent, Fluorescent Labeling Compound

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596105
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CAS #Purity
71418-44-5≥95% by HPLC

Precios y disponibilidad

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596105-25MG
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      Description
      OverviewHas been used to label E. coli thioredoxin. Weakly fluorescent but reacts with thiols to yield highly fluorescent stable thioethers.
      Catalogue Number596105
      Brand Family Calbiochem®
      SynonymsTHIOLYTE® MB Reagent, MBB
      References
      ReferencesMansoor, M.A., et al. 1992. Anal. Biochem. 200, 218.
      Zangerle, L., et al. 1992. J. Neurochem. 59, 181.
      Agarwal, R. and Burke, M. 1991. Arch. Biochem. Biophys. 290, 1.
      Martin, R.L., et al. 1989. J. Biol. Chem. 264, 11768.
      Bonet, A., et al. 1988. Biochem. Biophys. Res. Commun. 152, 1.
      Chinn, P.C. 1985. Fed. Proc. 44, 1620.
      Product Information
      CAS number71418-44-5
      FormYellow solid
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C. Aqueous stock solutions have a half-life of ~4 h at room temperature (20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      596105-25MG 07790788051907

      Documentation

      THIOLYTE® Monobromobimane Reagent, Fluorescent Labeling Compound Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      THIOLYTE® Monobromobimane Reagent, Fluorescent Labeling Compound Certificados de análisis

      CargoNúmero de lote
      596105

      Referencias bibliográficas

      Visión general referencias
      Mansoor, M.A., et al. 1992. Anal. Biochem. 200, 218.
      Zangerle, L., et al. 1992. J. Neurochem. 59, 181.
      Agarwal, R. and Burke, M. 1991. Arch. Biochem. Biophys. 290, 1.
      Martin, R.L., et al. 1989. J. Biol. Chem. 264, 11768.
      Bonet, A., et al. 1988. Biochem. Biophys. Res. Commun. 152, 1.
      Chinn, P.C. 1985. Fed. Proc. 44, 1620.
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-April-2008 RFH
      SynonymsTHIOLYTE® MB Reagent, MBB
      DescriptionContains a reactive functional group (allylic bromide) that is capable of reacting with a wide variety of nucleophiles to form stable covalent adducts. Reacts extremely rapidly with thiol groups under physiological conditions to yield highly fluorescent products. A labeling reaction is typically carried out at room temperature for 2 to 60 min, pH 7.4, using an equivalent amount (or a small excess) of reagent relative to the amount of thiol. Material is chemically and photolytically stable and shows an absorption maxima at 370 to 385 nm and emits light at 477 to 484 nm (turquoise). Readily penetrates membranes. Useful as an alternative tool in labeling methodology, complementing the use of such well known fluorescent labeling reagents as fluorescein and rhodamine isothiocyanates (whose reactions are slower and yield products that fade relatively fast during irradiation) and fluorescamine (which reacts primarily with amine groups, but whose products also fade relatively rapidly).
      FormYellow solid
      CAS number71418-44-5
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityAcetonitrile (13 mg/ml) or Methanol (25 mg/ml). Recommended stock solution is 13 mg/ml (50 mM) in acetonitrile.
      Storage Protect from light
      +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C. Aqueous stock solutions have a half-life of ~4 h at room temperature (20°C).
      Toxicity Standard Handling
      ReferencesMansoor, M.A., et al. 1992. Anal. Biochem. 200, 218.
      Zangerle, L., et al. 1992. J. Neurochem. 59, 181.
      Agarwal, R. and Burke, M. 1991. Arch. Biochem. Biophys. 290, 1.
      Martin, R.L., et al. 1989. J. Biol. Chem. 264, 11768.
      Bonet, A., et al. 1988. Biochem. Biophys. Res. Commun. 152, 1.
      Chinn, P.C. 1985. Fed. Proc. 44, 1620.