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329871 Erythropoietin, Human, Recombinant, CHO Cells

Erythropoietin, Human, Recombinant, CHO Cells, CAS 11096-26-7, is a glycoprotein with a predicted molecular weight of 21 kDa, but migrates at ~37 kDa on SDS-PAGE.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: EPO, Erythropoietin-α

329871
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Replacement Information

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329871-50UG
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      Description
      OverviewRecombinant, human erythropoietin (EPO) expressed in CHO cells. EPO is a glycoprotein produced by the kidneys and is involved in red blood cell production. It has a predicted molecular mass of ~21 kDa but migrates at ~37 kDa on SDS-PAGE.
      Catalogue Number329871
      Brand Family Calbiochem®
      SynonymsEPO, Erythropoietin-α
      References
      ReferencesFrank, S.J. 2002. Endocrinology 143, 2.
      Cerami, A. 2001. Semin. Hematol. 38, 33.
      Donnelly, S. 2001. Am. J. Kidney Dis. 38, 415.
      Goodnough, L.T. 2001. Curr. Opin. Hematol. 8, 405.
      Jelkmann, W, and Hellwig-Burgel, T. 2001. Adv. Exp. Med. Biol. 502, 169.
      Siren, A.L., and Ehrenreich, H. 2001. Eur. Arch. Psychiatry Clin. Neurosci 251, 179.
      Hansson, G.K., et al. 1992. Scand. J. Clin. Lab. Invest. 52, 35.
      Lee, L.G., et al. 1986. Cytometry 7, 35.
      Product Information
      CAS number11096-26-7
      FormLiquid
      FormulationIn 300 mM NaCl, 20 mM Na/K Phosphate, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥98% by HPLC
      Specific Activity≥1 x 10⁵ IU/mg
      Physicochemical Information
      ContaminantsEndotoxin: <10 EU/10 µg protein, EPO-α (1EU/µg)
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSKF5885000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C, up to 3 months at -20°C, or up to 6 months at -70°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      329871-50UG 04055977195682

      Documentation

      Erythropoietin, Human, Recombinant, CHO Cells Certificados de análisis

      CargoNúmero de lote
      329871

      Referencias bibliográficas

      Visión general referencias
      Frank, S.J. 2002. Endocrinology 143, 2.
      Cerami, A. 2001. Semin. Hematol. 38, 33.
      Donnelly, S. 2001. Am. J. Kidney Dis. 38, 415.
      Goodnough, L.T. 2001. Curr. Opin. Hematol. 8, 405.
      Jelkmann, W, and Hellwig-Burgel, T. 2001. Adv. Exp. Med. Biol. 502, 169.
      Siren, A.L., and Ehrenreich, H. 2001. Eur. Arch. Psychiatry Clin. Neurosci 251, 179.
      Hansson, G.K., et al. 1992. Scand. J. Clin. Lab. Invest. 52, 35.
      Lee, L.G., et al. 1986. Cytometry 7, 35.
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-March-2022 JSW
      SynonymsEPO, Erythropoietin-α
      DescriptionRecombinant, human erythropoietin (EPO) expressed in CHO cells. EPO is a glycoprotein hormone produced by the kidneys that is involved in the survival, growth, and differentiation of erythroid precursor cells. Stimulation of erythropoiesis involves the binding of EPO to the extracellular domain of the EPO receptor (EPOR) and triggering of intracellular signaling events, including phosphorylation of the receptor and activation of the JAK-STAT, RAS, and PI3-kinase pathways. Reported to act as a neuroprotective agent. Expressed in and purified from CHO cells. The predicted molecular weight is ~21 kDa, but it migrates at ~37 kDa on SDS-PAGE. Sialic acid content: > 10 moles/mole of protein. Sialic acid content: > 10 moles/mole of protein.
      FormLiquid
      FormulationIn 300 mM NaCl, 20 mM Na/K Phosphate, pH 7.5.
      CAS number11096-26-7
      RTECSKF5885000
      Purity≥98% by HPLC
      ContaminantsEndotoxin: <10 EU/10 µg protein, EPO-α (1EU/µg)
      Specific activity≥1 x 10⁵ IU/mg
      SolubilityReconstitute with 500 µl H₂O or aqueous buffer.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C, up to 3 months at -20°C, or up to 6 months at -70°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      Merck USA index14, 3688
      ReferencesFrank, S.J. 2002. Endocrinology 143, 2.
      Cerami, A. 2001. Semin. Hematol. 38, 33.
      Donnelly, S. 2001. Am. J. Kidney Dis. 38, 415.
      Goodnough, L.T. 2001. Curr. Opin. Hematol. 8, 405.
      Jelkmann, W, and Hellwig-Burgel, T. 2001. Adv. Exp. Med. Biol. 502, 169.
      Siren, A.L., and Ehrenreich, H. 2001. Eur. Arch. Psychiatry Clin. Neurosci 251, 179.
      Hansson, G.K., et al. 1992. Scand. J. Clin. Lab. Invest. 52, 35.
      Lee, L.G., et al. 1986. Cytometry 7, 35.