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203350 Blasticidin S, Hydrochloride, Streptomyces griseochromogenes - CAS 589205 - Calbiochem

This product can no longer be ordered through emdmillipore.com. Please refer to Cat. No. 203350 on sigmaaldrich.com.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Primary Target: protein synthesis
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203350
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Replacement Information

Tabla espec. clave

CAS #Empirical Formula
3513-03-9C₁₇H₂₆N₈O5 · HCl

Precios y disponibilidad

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203350-25MG
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      Description
      OverviewNucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. Suitable for use as a dominant selectable marker in conjunction with blasticidin S resistant plasmids. Blasticidin S resistance is conferred by the blasticidin S deaminase gene (bcr), which converts blastisidin S to a nontoxic deaminohydroxy derivative.
      Catalogue Number203350
      Brand Family Calbiochem®
      References
      ReferencesKimura, M., et al. 1994. Biochim. Biophys. Acta 1219, 653.
      Kimura, M., et al. 1994, Mol. Gen. Genet. 242, 121.
      Kojima, N., et al. 1994. J. Biol. Chem. 269, 30451.
      Kudo, T., et al. 1994. J. Immunol. Methods 177, 17.
      Sutoh, K. 1993. Plasmid 30, 150.
      Izumi, M., et al. 1991. Exp. Cell Res. 197, 229.
      Yamaguchi, H., et al. 1965. J. Biol. Chem. 57, 667.
      Takeuchi, S., et al. 1958. J. Antibiot. 11, 1.
      Product Information
      CAS number3513-03-9
      FormWhite solid
      Hill FormulaC₁₇H₂₆N₈O5 · HCl
      Chemical formulaC₁₇H₂₆N₈O5 · HCl
      Hygroscopic Hygroscopic
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Targetprotein synthesis
      Purity≥ 98% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 28

      Very toxic if swallowed.
      S PhraseS: 24/25-36/37-45

      Avoid contact with skin and eyes.
      Wear suitable protective clothing and gloves.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Highly Toxic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +2°C to +8°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Aqueous stock solutions (pH ≤7) are stable for up to 1 year at -20°C. Avoid alkaline pH. Do not subject to freeze/thaw cycles; upon thawing any aliquots, discard the unused portion.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      203350-25MG 07790788048341

      Documentation

      Blasticidin S, Hydrochloride, Streptomyces griseochromogenes - CAS 589205 - Calbiochem Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Blasticidin S, Hydrochloride, Streptomyces griseochromogenes - CAS 589205 - Calbiochem Certificados de análisis

      CargoNúmero de lote
      203350

      Referencias bibliográficas

      Visión general referencias
      Kimura, M., et al. 1994. Biochim. Biophys. Acta 1219, 653.
      Kimura, M., et al. 1994, Mol. Gen. Genet. 242, 121.
      Kojima, N., et al. 1994. J. Biol. Chem. 269, 30451.
      Kudo, T., et al. 1994. J. Immunol. Methods 177, 17.
      Sutoh, K. 1993. Plasmid 30, 150.
      Izumi, M., et al. 1991. Exp. Cell Res. 197, 229.
      Yamaguchi, H., et al. 1965. J. Biol. Chem. 57, 667.
      Takeuchi, S., et al. 1958. J. Antibiot. 11, 1.

      Folleto

      Cargo
      Antibiotics Profiler

      Citas

      Título
    • Richard L Bennett, et al. (2006) RAX, the PKR activator, sensitizes cells to inflammatory cytokines, serum withdrawal, chemotherapy and viral infection.. Blood in press,.
      		•
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-September-2019 JSW
      DescriptionNucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. Suitable for selection of cells carrying plasmids conferring blasticidin resistance. Blasticidin resistance is conferred by the blasticidin S deaminase genes (bsr from Bacillus cereus or BSD from Aspergillus terreus), whose products convert blasticidin S to a non-toxic deaminohydroxy derivative.
      FormWhite solid
      Recommended reaction conditionsTypically used at 2-10 µg/ml for mammalian cell selection.
      CAS number3513-03-9
      Chemical formulaC₁₇H₂₆N₈O5 · HCl
      Structure formulaStructure formula
      Purity≥ 98% by HPLC
      SolubilityH₂O (5 mg/ml) or Dilute Acetic Acid
      Storage +2°C to +8°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Aqueous stock solutions (pH ≤7) are stable for up to 1 year at -20°C. Avoid alkaline pH. Do not subject to freeze/thaw cycles; upon thawing any aliquots, discard the unused portion.
      Toxicity Highly Toxic
      Merck USA index14, 1316
      ReferencesKimura, M., et al. 1994. Biochim. Biophys. Acta 1219, 653.
      Kimura, M., et al. 1994, Mol. Gen. Genet. 242, 121.
      Kojima, N., et al. 1994. J. Biol. Chem. 269, 30451.
      Kudo, T., et al. 1994. J. Immunol. Methods 177, 17.
      Sutoh, K. 1993. Plasmid 30, 150.
      Izumi, M., et al. 1991. Exp. Cell Res. 197, 229.
      Yamaguchi, H., et al. 1965. J. Biol. Chem. 57, 667.
      Takeuchi, S., et al. 1958. J. Antibiot. 11, 1.
      Citation
    • Richard L Bennett, et al. (2006) RAX, the PKR activator, sensitizes cells to inflammatory cytokines, serum withdrawal, chemotherapy and viral infection.. Blood in press,.
      		•