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AB1541 Anti-Tryptophan Hydroxylase Antibody

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AB1541
100 µL  
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      Descripción

      Replacement Information
      Replacement InformationAB1541 is a recommended replacement for AB1556

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIHC, WBShAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1541
      ReplacesAB1556
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tryptophan Hydroxylase Antibody
      Alternate Names
      • Tryptophan 5-monooxygenase 1
      • indoleacetic acid-5-hydroxylase
      • tryptophan 5-hydroxylase
      • tryptophan 5-monooxygenase
      • tryptophan hydroxylase (tryptophan 5-monooxygenase)
      • tryptophan hydroxylase 1
      Background InformationTryptophan hydroxylase (TPH) is an enzyme involved in the synthesis of the neurotransmitter serotonin. It is responsible for addition of the -OH group (hydroxylation) to the 5 position to form the amino acid 5-hydroxytryptophan (5-HTP), which is the initial and rate-limiting step in the synthesis of the hormone serotonin. It is also the first enzyme in the synthesis of melatonin.
      Product Information
      FormatAffinity Purified
      Control
      • Brain tissue
      • PC12 lysates
      PresentationPurified sheep polyclonal liquid in 150 mM NaCl, 10 mM HEPES (pH 7.5), 100 μg/mL BSA and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationDetect Tryptophan Hydroxylase using this Anti-Tryptophan Hydroxylase Antibody validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry(peroxidase):
      Performed on formaldehyde fixed tissue of a previous lot. Useful for cell body but not fiber staining.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostSheep
      SpecificityRecognizes Tryptophan Hydroxylase. Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species. Recently studies have indicated that AB1541 can cross react with Tyrosine hydroxylase on western blots, weakly. Cross reactivity in the native state is untested.
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteReacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the gut, pineal gland, spleen, and thymus.
      Gene Symbol
      • TPH1
      • TPRH
      • MGC119994
      • TRPH
      • TPH
      • EC 1.14.16.4
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryCOFACTOR:Fe(2+) ion.
      SIZE:444 amino acids; 50985 Da
      SUBUNIT:Multimer of identical subunits.
      TISSUE SPECIFICITY:Isoform 2 seems to be less widely expressed than isoform 1.
      SIMILARITY:SwissProt: P17752 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. & Contains 1 ACT domain.
      Molecular Weight55 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected Tryptophan Hydroxylase on 10 μg of PC12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20ºC to -80ºC in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Número de referencia GTIN
      AB1541 04053252626227